Locus  

Presence of Mycobacterium avium subsp. paratuberculosis (MAP) in Brazilian patients with inflammatory bowel diseases and in controls

Show simple item record

dc.contributor.author Carvalho, Isabel Azevedo
dc.contributor.author Schwarz, David Germano Gonçalves
dc.contributor.author Pietralonga, Pricila Aparecida Grasse
dc.contributor.author Faria, Ana Carolina Silva
dc.contributor.author Braga, Isis Freitas Espechit
dc.contributor.author Carvalho, Gabriel Domingos
dc.contributor.author Valente, Fabrício Luciani
dc.contributor.author Machado, João Paulo
dc.contributor.author Guimarães, Lize Maciel Pinheiro
dc.contributor.author Ferrari, Maria de Lourdes Abreu
dc.contributor.author Júnior, Abelardo Silva
dc.contributor.author Moreira, Maria Aparecida Scatamburlo
dc.date.accessioned 2017-10-17T15:56:17Z
dc.date.available 2017-10-17T15:56:17Z
dc.date.issued 2014-09-18
dc.identifier.issn 18069460
dc.identifier.uri http://dx.doi.org/10.1590/1516-3180.2014.8251809
dc.identifier.uri http://www.locus.ufv.br/handle/123456789/12105
dc.description.abstract Mycobacterium avium subsp. paratuberculosis (MAP) has attracted the interest of researchers because of similarities between paratuberculosis and Crohn's disease (CD). The aim of this study was to evaluate the frequency of MAP through cultures, histology and polymerase chain reaction (PCR) on intestinal biopsies from Brazilian CD patients. Quantitative real time PCR (qRT-PCR) was performed on positive samples. Fresh samples were collected from 25 patients; five with CD, eight with ulcerative colitis (UC) and 12 controls with non-inflammatory bowel disease (nIBD). Formalin-fixed paraffin-embedded (FFPE) samples from 143 patients were also collected: 44 CD, 49 UC and 56 nIBD. None of the fresh samples was positive for MAP. Five FFPE samples (one CD, two UC and two nIBD) and three fresh samples (one in each group) were positive through IS900-PCR. qRT-PCR was performed on these eight samples. Among the FFPE samples, there were 192.12 copies/μl in the CD group, 72.28 copies/μl in UC and 81.43 copies/μl in nIBD. Among the fresh samples, there were 432.99 copies/μl, 167.92 copies/μl and 249.73 copies/μl in the CD, UC and nIBD groups, respectively. The highest bacterial load was in the CD group. This study does not provide evidence for a role of MAP in the etiology of CD, although MAP DNA was detected in all three patient groups. This is the first report of MAP presence in human intestinal biopsies in Brazil. en
dc.description.abstract Mycobaterium avium subsp. paratuberculosis (MAP) tem atraído o interesse de pesquisadores devido às semelhanças entre a paratuberculose e a doença de Crohn (CD). Este estudo objetivou avaliar a frequência de MAP por meio de cultura, histologia e reação da polimerase em cadeia (PCR), em biópsias intestinais de pacientes brasileiros com CD. PCR quantitativa em tempo real (qRT-PCR) foi realizada nas amostras positivas. Estudo transversal analítico com grupo controle realizado em duas universidades federais. Amostras frescas foram coletadas de 25 pacientes; cinco com CD, oito com colite ulcerativa (UC) e 12 controles sem doença inflamatória intestinal (nIBD). Também foram coletadas 149 amostras fixadas em parafina (FFPE): 44 CD, 49 UC e 56 nIBD. Nenhuma das amostras frescas foi positiva para MAP. Cinco amostras FFPE (uma CD, duas UC e duas nIBD) e três amostras frescas (uma de cada grupo) foram positivas por IS900-PCR. qRT-PCR foi realizada nessas oito amostras. Nas amostras FFPE, havia 192,12 cópias/μl no grupo CD, 72,28 cópias/μl no UC e 81,43 cópias/μl no nIBD. Nas amostras frescas, havia 432,99 cópias/μl, 167,92 cópias/μl e 249,73 cópias/μl nos grupos CD, UC e nIBD, respectivamente. A maior carga bacteriana foi encontrada no grupo CD. Este estudo não fornece evidências do papel de MAP na etiologia da CD, embora DNA de MAP tenha sido detectado em pacientes dos três grupos. Este é o primeiro relato da presença de MAP em biópsias intestinais humanas no Brasil. pt-BR
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Sao Paulo Medical Journal pt-BR
dc.relation.ispartofseries vol.134 n.1, 13-9, julho 2015 pt-BR
dc.rights Open Access pt-BR
dc.subject Mycobacterium avium subsp pt-BR
dc.subject Paratuberculosis pt-BR
dc.subject Crohn disease pt-BR
dc.subject Inflammatory bowel diseases pt-BR
dc.subject Colitis pt-BR
dc.subject Ulcerative pt-BR
dc.subject Brazil pt-BR
dc.title Presence of Mycobacterium avium subsp. paratuberculosis (MAP) in Brazilian patients with inflammatory bowel diseases and in controls en
dc.type Artigo pt-BR


Files in this item

This item appears in the following Collection(s)

  • Artigos [537]
    Artigos Técnico-científicos na área de Veterinária

Show simple item record

Search DSpace


Browse

My Account