Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/12210
Tipo: Artigo
Título: Schinus terebinthifolius leaf extract causes midgut damage, interfering with survival and development of Aedes aegypti larvae
Autor(es): Procópio, Thamara Figueiredo
Fernandes, Kenner Morais
Pontual, Emmanuel Viana
Ximenes, Rafael Matos
Oliveira, Aline Rafaella Cardoso de
Souza, Carolina de Santana
Melo, Ana Maria Mendonça de Albuquerque
Navarro, Daniela Maria do Amaral Ferraz
Paiva, Patrícia Maria Guedes
Martins, Gustavo Ferreira
Napoleão, Thiago Henrique
Abstract: In this study, a leaf extract from Schinus terebinthifolius was evaluated for effects on survival, development, and midgut of A. aegypti fourth instar larvae (L4), as well as for toxic effect on Artemia salina. Leaf extract was obtained using 0.15 M NaCl and evaluated for phytochemical composition and lectin activity. Early L4 larvae were incubated with the extract (0.3–1.35%, w/v) for 8 days, in presence or absence of food. Polymeric proanthocyanidins, hydrolysable tannins, heterosid and aglycone flavonoids, cinnamic acid derivatives, traces of steroids, and lectin activity were detected in the extract, which killed the larvae at an LC50 of 0.62% (unfed larvae) and 1.03% (fed larvae). Further, the larvae incubated with the extract reacted by eliminating the gut content. No larvae reached the pupal stage in treatments at concentrations between 0.5% and 1.35%, while in the control (fed larvae), 61.7% of individuals emerged as adults. The extract (1.0%) promoted intense disorganization of larval midgut epithelium, including deformation and hypertrophy of cells, disruption of microvilli, and vacuolization of cytoplasms, affecting digestive, enteroendocrine, regenerative, and proliferating cells. In addition, cells with fragmented DNA were observed. Separation of extract components by solid phase extraction revealed that cinnamic acid derivatives and flavonoids are involved in larvicidal effect of the extract, being the first most efficient in a short time after larvae treatment. The lectin present in the extract was isolated, but did not show deleterious effects on larvae. The extract and cinnamic acid derivatives were toxic to A. salina nauplii, while the flavonoids showed low toxicity. S. terebinthifolius leaf extract caused damage to the midgut of A. aegypti larvae, interfering with survival and development. The larvicidal effect of the extract can be attributed to cinnamic acid derivatives and flavonoids. The data obtained using A. salina indicates that caution should be used when employing this extract as a larvicidal agent.
Palavras-chave: Schinus terebinthifolius
Aedes aegypti
Editor: PLoS One
Tipo de Acesso: Open Access
URI: https://doi.org/10.1371/journal.pone.0126612
http://www.locus.ufv.br/handle/123456789/12210
Data do documento: 14-Mai-2015
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