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Hydrolysis of soybean isoflavones by Debaryomyces hansenii UFV-1 immobilised cells and free β-glucosidase

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dc.contributor.author Maitan-Alfenas, Gabriela P.
dc.contributor.author Lage, Lorena G. de A.
dc.contributor.author Almeida, Maíra N. de
dc.contributor.author Visser, Evan M.
dc.contributor.author Rezende, Sebastião T. de
dc.contributor.author Guimarães, Valéria M.
dc.date.accessioned 2017-10-26T11:13:36Z
dc.date.available 2017-10-26T11:13:36Z
dc.date.issued 2014-03-01
dc.identifier.issn 0308-8146
dc.identifier.uri https://doi.org/10.1016/j.foodchem.2013.09.099
dc.identifier.uri http://www.locus.ufv.br/handle/123456789/12419
dc.description.abstract An intracellular β-glucosidase from Debaryomyces hansenii UFV-1 was produced in an YP medium with cellobiose as the carbon source. This enzyme was purified, characterised and presented a Mr of 65.15 kDa. Yeast cells containing the intracellular β-glucosidase were immobilised in calcium alginate. The free β-glucosidase and immobilised cells containing the enzyme presented optima values of pH and temperature of 6.0 and 45 °C and 5.5 and 50 °C, respectively. The free enzyme maintained 62% and 47% of its original activity after 90 days at 4 °C and after 15 days at room temperature, respectively. The immobilisation process resulted in higher enzyme thermostability at 45 and 50 °C. Soy molasses treatment with the free enzyme and the immobilised cells containing β-glucosidase, for 2 h at 40 °C, promoted efficient hydrolysis of isoflavone glicosides to their aglycon forms. The results suggest that this enzyme could be used in the food industry, in the free or immobilised forms, for a safe and efficient process to hydrolyse isoflavone glycosides in soy molasses. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Food Chemistry pt-BR
dc.relation.ispartofseries Volume 146, Pages 429-436, March 2014 pt-BR
dc.rights Open Access pt-BR
dc.subject Debaryomyces hansenii UFV-1 pt-BR
dc.subject β-Glucosidase pt-BR
dc.subject Immobilisation pt-BR
dc.subject Isoflavones pt-BR
dc.subject Soy molasses pt-BR
dc.title Hydrolysis of soybean isoflavones by Debaryomyces hansenii UFV-1 immobilised cells and free β-glucosidase en
dc.type Artigo pt-BR


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  • Artigos [261]
    Artigos Técnico-científicos nas áreas de Bioquímica e Biologia Molecular

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