Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/12448
Tipo: Artigo
Título: Impaired malate and fumarate accumulation due the mutation of tonoplast dicarboxylate transporter
Autor(es): Medeiros, David B.
Barros, Kallyne
Barros, Jessica AS
Omena-Garcia, Rebeca P
Detmann, Kelly C
Silva, Willian Batista
DaMatta, Fabio
Nunes-Nesi, Adriano
Araújo, Wagner L.
Arrivault, Stéphanie
Sanglard, Lilian Vincis Pereira
Daloso, Danilo M.
Fernie, Alisdair R.
Abstract: Malate is a central metabolite involved in a multiplicity of plant metabolic pathways, being associated with mitochondrial metabolism and playing significant roles n stomatal movements. Vacuolar malate transport has been characterized at the molecular level and is performed by at least one carrier protein and two channels in Arabidopsis vacuoles. The absence of the Arabidopsis thaliana tonoplast Dicarboxylate Transporter (tDT) in tdt knockout mutant was previously associated with an impaired accumulation of malate and fumarate in leaves. Here, we investigated the consequences of this lower accumulation on stomatal behaviour and photosynthetic capacity as well as ts putative metabolic impacts. Neither the stomatal conductance (g s ) nor the kinetic responses to dark, light or high CO 2 were highly affected in tdt plants. In addition, we did not observe any impact on stomatal aperture following incubation with either bscisic acid (ABA), malate or citrate. Further, an effect on photosynthetic capacity was not observed in the mutant lines. However, leaf mitochondrial metabolism was affected in the tdt plants. Levels of the intermediates of the tricarboxylic acid cycle were altered and increases in both light and dark respiration were observed. We conclude that manipulation of the tonoplastic organic acid transporter impacted mitochondrial metabolism, while the overall stomatal and photosynthetic capacity were unaffected.
Palavras-chave: Organic acids
Growth
Primary metabolism
Respiration
Stomata
Editor: Plant Physiology
Tipo de Acesso: Open Acess
URI: https://doi.org/10.1104/pp.17.00971
http://www.locus.ufv.br/handle/123456789/12448
Data do documento: 20-Set-2017
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
pp.17.00971.full.pdftexto completo1,06 MBAdobe PDFThumbnail
Visualizar/Abrir


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.