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C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae)

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dc.contributor.author Maffei, Eliane Mariza Dortas
dc.contributor.author Pompolo, Silvia das Graças
dc.contributor.author Petitpierre, Eduard
dc.date.accessioned 2017-10-30T09:28:50Z
dc.date.available 2017-10-30T09:28:50Z
dc.date.issued 2003-11-14
dc.identifier.issn 1678-4685
dc.identifier.uri http://dx.doi.org/10.1590/S1415-47572004000200011
dc.identifier.uri http://www.locus.ufv.br/handle/123456789/12474
dc.description.abstract The aim of this study was to describe mitotic and meiotic chromosomes of Cycloneda sanguinea using C-banding, fluorescent in situ hybridization (FISH) rDNA probes, and sequential FISH/Ag-NOR staining. The chromosome number was 2n = 18 + XX for females and 2n = 18 + Xy for males. The X chromosome was metacentric and the Y chromosome was very small. During meiosis, the karyotypic meioformula was n = 9 + Xyp, and sex chromosomes configured a parachute at metaphase I. At the beginning of pachytene, bivalents were still individualized, and sex chromosomes were associated end-to-end through the heteropycnotic region of the X chromosome. Later in pachytene, further condensation led to the formation of a pseudo-ring by the sex bivalent. All chromosomes showed pericentromeric heterochromatin. FISH and sequential FISH/Ag-NOR staining evidenced the location of the nucleolar organizer region in one pair of autosomes (at spermatogonial metaphase). During meiosis, these genes were mapped to a region outside the sex vesicle by FISH, although Xyp was deeply stained with silver at metaphase I. These results suggest that these argyrophilic substances are of a nucleolar protein nature, and seem to be synthesized by a pair of autosomes and imported during meiosis (prophase I) to the sex pair, during the association of the sex chromosomes. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Genetics and Molecular Biology pt-BR
dc.relation.ispartofseries 27(2), p.191-195, 2004 pt-BR
dc.rights Open Access pt-BR
dc.subject In situ hybridization pt-BR
dc.subject rDNA sequences pt-BR
dc.subject Sequential FISH/Ag-NOR staining pt-BR
dc.subject C-banding pt-BR
dc.subject Cycloneda sanguinea. pt-BR
dc.title C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae) en
dc.type Artigo pt-BR


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  • Artigos [660]
    Artigos Técnico-científicos na área de Biologia Geral

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