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2,6-Diaminopimelic acid (DAPA) in microbial protein quantification of heifers fed different forage sources

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dc.contributor.author Couto Filho, Cristovão Colombo de Carvalho
dc.contributor.author Saliba, Eloísa de Oliveira Simões
dc.contributor.author Rodriguez, Norberto Mario
dc.contributor.author Barbosa, Geraldo Sérgio Senra Carneiro
dc.contributor.author Freitas, Regeane Martins de
dc.contributor.author Resende, Matheus Pinheiro Diniz
dc.date.accessioned 2017-11-30T09:27:25Z
dc.date.available 2017-11-30T09:27:25Z
dc.date.issued 2014-06-23
dc.identifier.issn 1806-9290
dc.identifier.uri http://dx.doi.org/10.1590/S1806-92902015000500003
dc.identifier.uri http://www.locus.ufv.br/handle/123456789/14035
dc.description.abstract The objective of this study was to evaluate the flow of nitrogenous compounds, protein degradability, rumen degradation of total carbohydrate and organic matter and microbial efficiency in heifers subjected to diets containing corn silage, sugarcane or Tifton. For this purpose, the 2,6-diaminopimelic acid (DAPA) technique was adopted and analytical procedures for amino acids by HPLC were adapted. Six rumen-fistulated Holstein-Zebu heifers with 480 kg of initial BW and at 24 months of age kept in individual tie stalls were assigned to two 3 × 3 Latin squares. Omasal digesta dry matter and microbial dry matter flows were determined using the isolated, purified and enriched lignin (LIPE®) and DAPA markers, respectively. Isolated bacteria from rumen showed on average 5.84 g/100 g microbial N, 0.25 g/100 g DAPA in dry matter and 44.61 DAPA:N ratio. The forage sources did not influence the flows of nitrogen compounds, except for total omasal flow and non-ammonia N in relation to N intake for the corn silage diet, for which there was an upward trend compared with the other diets. The degradation of the organic matter and total carbohydrates did not differ, averaging 6.1 kg/day and 5.2 kg/day, respectively. The studied forage sources do not influence the flows of nitrogen compounds, except for total omasal flow and non-ammonia N in relation N intake for the corn silage diet, for which there is an upward trend compared with the other diets. Protein degradability and microbial efficiency are similar between evaluated diets. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Revista Brasileira de Zootecnia pt-BR
dc.relation.ispartofseries v. 44, n. 5. p. 180-186, May 2015 pt-BR
dc.rights Open Access pt-BR
dc.subject Corn silage pt-BR
dc.subject Flow of nitrogen compounds pt-BR
dc.subject HPLC pt-BR
dc.subject Microbial efficiency pt-BR
dc.subject Protein degradability pt-BR
dc.subject Sugarcane pt-BR
dc.title 2,6-Diaminopimelic acid (DAPA) in microbial protein quantification of heifers fed different forage sources en
dc.type Artigo pt-BR


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  • Artigos [52]
    Artigos Técnico-científicos do Instituto de Ciências Agrárias

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