Use este identificador para citar ou linkar para este item:
https://locus.ufv.br//handle/123456789/18614
Tipo: | Artigo |
Título: | Antioxidant, antiproliferative, and immunostimulatory effects of cell wall α-d-mannan fractions from Kluyveromyces marxianus |
Autor(es): | Galinari, Éder Almeida-Lima, Jailma Macedo, Gorete Ribeiro Mantovani, Hilário Cuquetto Rocha, Hugo Alexandre Oliveira |
Abstract: | This study evaluated the antioxidant, antiproliferative, and immunostimulatory properties of cell wall α-d-mannan fractions from yeast Kluyveromyces marxianus CCT7735. Filter centrifugation was used to obtain four fractions (KMM-1, KMM-2, KMM-3, and KMM-4) with molecular weight ranging from 7.6 to 75.1 kDa. KMM-1 and KMM-2 comprised D-mannose with traces of D-glucose, whereas other fractions contained only D-mannose. Total sugar found in samples ranged from 85.9% to 96.1%, while protein and phenolic contents were 1.21% and 0.41%, respectively. Although only KMM-1 was able to scavenge superoxide radicals, all fractions presented total antioxidant capacity as well as reducing power, hydroxyl-radical scavenging, and copper- and iron-chelating activities. No fraction was cytotoxic to HeLa cells. However, all samples inhibited the proliferation of the tumor cell Hep-G2 and presented minor cytotoxicity against normal 3T3 cells. All fractions showed mitogenic activity in macrophages and all, except KMM-4, induced nitric oxide production in macrophages, suggestive of their immunostimulatory effects. |
Palavras-chave: | Copper-chelating Iron-chelating Mitogenic activity Cell wall polysaccharide Nitric oxide |
Editor: | International Journal of Biological Macromolecules |
Tipo de Acesso: | Elsevier B.V. All rights reserved. |
URI: | https://doi.org/10.1016/j.ijbiomac.2017.11.053 http://www.locus.ufv.br/handle/123456789/18614 |
Data do documento: | 11-Nov-2017 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo.pdf Until 2100-05-28 | Texto completo | 1,96 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.