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https://locus.ufv.br//handle/123456789/18912
Tipo: | Artigo |
Título: | Development of a transformation system for Crinipellis perniciosa, the causal agent of witches' broom in cocoa plants |
Autor(es): | Lima, Juliana Oliveira Santos, Jildete Karla dos Pereira, Jorge Fernando Resende, Mário Lúcio Vilela de Araújo, Elza Fernandes de Queiroz, Marisa Vieira de |
Abstract: | Protoplasts of the pathogenic plant fungus, Crinipellis perniciosa, were transformed to hygromycin B resistance using the pAN7-1 plasmid, which contains the Escherichia coli hph gene under the control of Aspergillus nidulans regulatory sequences. The pAN7-1 plasmid was introduced by PEG/CaCl2 treatment. Transformation frequencies of 1.6–2.5 transformants/μg of DNA were achieved. About 54% of the transformants were abortive and 40 analyzed transformants were mitotically stable and showed different hygromycin B resistance levels. The presence of the hph gene was checked by PCR in five transformants and the integration of multiple plasmid copies into different genome sites was observed by Southern analysis. This is the first report of a C. perniciosa transformation system and represents an important step for further research into genetic manipulation of this fungal plant pathogen. |
Palavras-chave: | Transformation Hygromycin resistance Witches' broom |
Editor: | Current Genetics |
Tipo de Acesso: | Springer-Verlag |
URI: | https://link.springer.com/article/10.1007/s00294-002-0358-z http://www.locus.ufv.br/handle/123456789/18912 |
Data do documento: | 17-Dez-2002 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 257,09 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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