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https://locus.ufv.br//handle/123456789/19344
Tipo: | Artigo |
Título: | Leishmania chagasi heparin-binding protein: Cell localization and participation in L. chagasi infection |
Autor(es): | Martins, Thaís Viana Fialho Carvalho, Thaís Vieira de Oliveira, Claudia Vânia Miranda de Paula, Sérgio Oliveira de Cardoso, Sílvia Almeida Oliveira, Leandro Licursi de Silva, Eduardo de Almeida Marques-da |
Abstract: | Visceral leishmaniasis is a fatal human disease caused by the intracellular protozoan parasite Leishmania chagasi that is captured by host cells in a process involving classics receptors mediated phagocytosis. The search for molecules involved in this process is important to design strategies to disease control. In this work, we verified the presence of heparin-binding protein (HBP) in L. chagasi promastigotes forms. HBP is a lectin of the group of ubiquitous proteins, whose main characteristic is to bind to carbohydrates present in glycoproteins or glycolipids, which is poorly studied in Leishmania species. L. chagasi HBP (HBPLc) was purified by affinity chromatography using heparin–agarose column in FPLC automated system. Its localization in the parasite was assessed by immunolabeling and electronic transmission microscopy tests using anti-HBPLc polyclonal antibodies, which showed HBP spread over the parasite outer surface and internally next to the kynetoplast. In addition, we verified that HBPLc participates in the process of parasite infection, since its blocking with heparin generated a partial reduction in the internalization of Leishmania by RAW macrophages “in vitro”. According to these results, it is believed that, in further “in vivo” studies, interference on this parasitic protein may provide us prophylactic and therapeutic alternatives against visceral leishmaniasis. |
Palavras-chave: | Leishmania Lectins Heparin Visceral leishmaniasis |
Editor: | Molecular and Biochemical Parasitology |
Tipo de Acesso: | Elsevier B.V. |
URI: | https://doi.org/10.1016/j.molbiopara.2015.12.005 http://www.locus.ufv.br/handle/123456789/19344 |
Data do documento: | 21-Dez-2015 |
Aparece nas coleções: | Artigos |
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Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 2,32 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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