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https://locus.ufv.br//handle/123456789/19763
Tipo: | Artigo |
Título: | Purification of the seven tetranortriterpenoids in neem (Azadirachta indica) seed by counter-current chromatography sequentially followed by isocratic preparative reversed-phase high-performance liquid chromatography |
Autor(es): | Silva, Júlio César T. Jham, Gulab N. Oliveira, Rosângela D’arc L. Brown, Leslie |
Abstract: | Counter-current chromatography (CCC) sequentially followed by isocratic preparative reversed-phase high-performance liquid chromatography was used to isolate the seven bio-actives (azadirachtin A, azadirachtin B, azadirachtin H, desacetylnimbin, desacetylsalannin, nimbin and salannin) from the seed concentrate (NSC) of the neem tree (Azadirachta indica A. Juss). Reproducible, narrow polarity range, high purity fractions were obtained from repeated injections of the NSC (700 mg loadings/injection), on to a relatively small volume CCC coil (116 mL). The CCC biphasic solvent system chosen was hexane:butanol:methanol:water (1:0.9:1:0.9, v/v). A mass balance of injected material showed that 95+% were recovered. |
Palavras-chave: | Neem Tetranortriterpenoids Limonoids Counter-current chromatography Preparative high-performance liquid chromatography |
Editor: | Journal of Chromatography A |
Tipo de Acesso: | Elsevier B.V. |
URI: | https://doi.org/10.1016/j.chroma.2007.03.086 http://www.locus.ufv.br/handle/123456789/19763 |
Data do documento: | 30-Mar-2007 |
Aparece nas coleções: | Artigos |
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artigo.pdf Until 2100-12-31 | Texto completo | 409,77 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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