Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/22323
Tipo: Artigo
Título: Kinetics and regulation of lactose transport and metabolism in Kluyveromyces lactis JA6
Autor(es): Santos, A. M.
Silveira, W. B.
Fietto, L. G.
Brandão, R. L.
Castro, I. M.
Abstract: Kluyveromyces lactis strains are able to assimilate lactose. They have been used industrially to eliminate this sugar from cheese whey and in other industrial products. In this study, we investigated specific features and the kinetic parameters of the lactose transport system in K. lactis JA6. In lactose grown cells, lactose was transported by a system transport with a half-saturation constant (K s) of 1.49 ± 0.38 mM and a maximum velocity (V max) of 0.96 ± 0.12 mmol. (g dry weight^)−1 h^−1 for lactose. The transport system was constitutive and energy-dependent. Results obtained by different approaches showed that the lactose transport system was regulated by glucose at the transcriptional level and by glucose and other sugars at a post-translational level. In K. lactis JA6, galactose metabolization was under glucose control. These findings indicated that the regulation of lactose-galactose regulon in K. lactis was similar to the regulation of galactose regulon in Saccharomyces cerevisiae.
Palavras-chave: Kluyveromyces
Lactose transport
Glucose control
Regulation
Editor: World Journal of Microbiology and Biotechnology
Tipo de Acesso: Springer Nature Switzerland AG.
URI: http://dx.doi.org/10.1007/s11274-014-1620-5
http://www.locus.ufv.br/handle/123456789/22323
Data do documento: 7-Fev-2014
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