Kinetics and regulation of lactose transport and metabolism in Kluyveromyces lactis JA6

Show simple item record Santos, A. M. Silveira, W. B. Fietto, L. G. Brandão, R. L. Castro, I. M. 2018-10-17T17:19:10Z 2018-10-17T17:19:10Z 2014-02-07
dc.identifier.issn 15730972
dc.description.abstract Kluyveromyces lactis strains are able to assimilate lactose. They have been used industrially to eliminate this sugar from cheese whey and in other industrial products. In this study, we investigated specific features and the kinetic parameters of the lactose transport system in K. lactis JA6. In lactose grown cells, lactose was transported by a system transport with a half-saturation constant (K s) of 1.49 ± 0.38 mM and a maximum velocity (V max) of 0.96 ± 0.12 mmol. (g dry weight^)−1 h^−1 for lactose. The transport system was constitutive and energy-dependent. Results obtained by different approaches showed that the lactose transport system was regulated by glucose at the transcriptional level and by glucose and other sugars at a post-translational level. In K. lactis JA6, galactose metabolization was under glucose control. These findings indicated that the regulation of lactose-galactose regulon in K. lactis was similar to the regulation of galactose regulon in Saccharomyces cerevisiae. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher World Journal of Microbiology and Biotechnology pt-BR
dc.relation.ispartofseries v. 30, n. 7, p. 1977– 1983, jul. 2014 pt-BR
dc.rights Springer Nature Switzerland AG. pt-BR
dc.subject Kluyveromyces pt-BR
dc.subject Lactose transport pt-BR
dc.subject Glucose control pt-BR
dc.subject Regulation pt-BR
dc.title Kinetics and regulation of lactose transport and metabolism in Kluyveromyces lactis JA6 en
dc.type Artigo pt-BR

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    Artigos Técnico-científicos na área de Microbiologia

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