Pectin lyase production by recombinant Penicillium griseoroseum strain 105

Show simple item record Teixeira, Janaina Aparecida Queiroz, Marisa Vieira de Araújo, Elza Fernandes de Cardoso, Patrícia Gomes 2019-01-11T18:39:57Z 2019-01-11T18:39:57Z 2010
dc.identifier.issn 1480-3275
dc.description.abstract Recombinant Penicillium griseoroseum strain 105 overproduces an extracellular pectin lyase (PL) under the transcriptional control of the strong gpdA promoter of Aspergillus nidulans. Our aim was to evaluate PL production by recombinant P. griseoroseum strain 105 in submerged fermentation system bioreactors BioFloIII and BioFloIV using 2 or 10 L working volumes under different growth conditions and to analyze the production of cellulase, polygalacturonase, pectin methylesterase, and protease. PL overproduction by recombinant P. griseoroseum strain 105 was 112 times higher than that of P. griseoroseum PG63 grown in sugarcane juice. Cellulases and proteases were not detected in the culture filtrate, and evaluation for extracellular proteins in the culture medium by SDS–PAGE showed the presence of a 36 kDa predominant band, similar to the molecular mass estimated from the nucleotide sequence of plg1 gene for PL of P. griseoroseum strain 105. This recombinant strain provides the advantage of PL production, which predominates over other extracellular proteins usually present in most commercial pectinase preparations, using sugarcane juice as a substrate of low cost. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Canadian Journal of Microbiology pt-BR
dc.relation.ispartofseries Volume 56, Issue 10, p. 831-837, 2010 pt-BR
dc.rights NRC Research Press pt-BR
dc.subject Penicillium griseoroseum pt-BR
dc.subject Recombinant strain pt-BR
dc.subject Fermentation pt-BR
dc.subject Pectin lyase pt-BR
dc.subject Enzyme stability pt-BR
dc.title Pectin lyase production by recombinant Penicillium griseoroseum strain 105 en
dc.type Artigo pt-BR

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    Artigos Técnico-científicos na área de Microbiologia

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