Use este identificador para citar ou linkar para este item:
https://locus.ufv.br//handle/123456789/23919
Tipo: | Artigo |
Título: | Variable ITS-copy number at different developmental stages of Meloidogyne hapla and M. chitwoodi |
Autor(es): | Lopes, Everaldo Antônio Roberts, David Mark Blok, Vivian Carol |
Abstract: | Quantitative polymerase chain reaction (qPCR) can be used for the accurate quantification of plant-parasitic nematodes from soil samples. Unlike the traditional Baermann funnel and centrifugal flotation techniques followed by visual enumeration, all developmental stages of plant-parasitic nematodes can be detected and quantified by using qPCR. However, little is known about the amount of DNA between different stages of plant-parasitic nematodes. Here, we show that ITS-copy number varies with developmental stage of Meloidogyne chitwoodi and M. hapla. The number of copies of ITS was lower in cell-stage eggs (51 ± 7.4 for M. hapla and 31 ± 6.0 for M. chitwoodi) than in second-stage juveniles (1345 ± 130.6 for M. hapla and 1036 ± 140.3 for M. chitwoodi) and females (19,508 ± 3367.3 for M. hapla and 9049 ± 316.7 for M. chitwoodi). |
Palavras-chave: | Number of copies qPCR Quantification Real-time PCR Root-knot nematode |
Editor: | European Journal of Plant Pathology |
Tipo de Acesso: | Koninklijke Nederlandse Planteziektenkundige Vereniging |
URI: | https://doi.org/10.1007/s10658-019-01672-8 http://www.locus.ufv.br/handle/123456789/23919 |
Data do documento: | 2019 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo.pdf Until 2100-12-31 | Texto completo | 473,62 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.