Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/23919
Tipo: Artigo
Título: Variable ITS-copy number at different developmental stages of Meloidogyne hapla and M. chitwoodi
Autor(es): Lopes, Everaldo Antônio
Roberts, David Mark
Blok, Vivian Carol
Abstract: Quantitative polymerase chain reaction (qPCR) can be used for the accurate quantification of plant-parasitic nematodes from soil samples. Unlike the traditional Baermann funnel and centrifugal flotation techniques followed by visual enumeration, all developmental stages of plant-parasitic nematodes can be detected and quantified by using qPCR. However, little is known about the amount of DNA between different stages of plant-parasitic nematodes. Here, we show that ITS-copy number varies with developmental stage of Meloidogyne chitwoodi and M. hapla. The number of copies of ITS was lower in cell-stage eggs (51 ± 7.4 for M. hapla and 31 ± 6.0 for M. chitwoodi) than in second-stage juveniles (1345 ± 130.6 for M. hapla and 1036 ± 140.3 for M. chitwoodi) and females (19,508 ± 3367.3 for M. hapla and 9049 ± 316.7 for M. chitwoodi).
Palavras-chave: Number of copies
qPCR
Quantification
Real-time
PCR
Root-knot nematode
Editor: European Journal of Plant Pathology
Tipo de Acesso: Koninklijke Nederlandse Planteziektenkundige Vereniging
URI: https://doi.org/10.1007/s10658-019-01672-8
http://www.locus.ufv.br/handle/123456789/23919
Data do documento: 2019
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