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https://locus.ufv.br//handle/123456789/24375
Tipo: | Artigo |
Título: | De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production |
Autor(es): | Koehler, Andréa Dias Silva, Tatiane Dulcineia Almeida, Maíra Carolina Ferreira, Perácio Rafael Bueno Felipe, Sérgio Heitor Sousa Dias, Leonardo Lucas Carnevalli Otoni, Wagner Campos Maciel, Talles Elisson F. Romanel, Elisson Saldanha, Cleber Witt Souza, Vinícius Cairus de Maldaner, Joseila Dias, Leonardo Lucas Carnevalli Festucci-Buselli, Reginaldo Alves |
Abstract: | Pfaffia glomerata is a medically important species because it produces the phytoecdysteroid 20-hydroxyecdysone (20-E). However, there has been no ready-to-use transcriptome data available in the literature for this plant. Here, we present de novo transcriptome sequencing of RNA from P. glomerata in order to investigate the 20-E production as well as to understand the biochemical pathway of secondary metabolites in this non-model species. We then analyze the effect of photoautotrophy on the production of 20-E genes phylogenetically identified followed by expression analysis. For this, total messenger RNA (mRNA) from leaves, stems, roots, and flowers was used to construct indexed mRNA libraries. Based on the similarity searches against plant non-redundant protein database, gene ontology, and eukaryotic orthologous groups, 164,439 transcripts were annotated. In addition, the effect of photoautotrophy in two genes putatively involved in the 20-E synthesis pathway was analyzed. The Phantom gene (CYP76C), a precursor of the route, showed increased expression in P. glomerata plants cultured under photoautotrophic conditions. This was accompanied by increased production of this metabolite indicating a putative involvement in 20-E synthesis. This work reveals that several genes in the P. glomerata transcriptome are related to secondary metabolism and stresses, that genes of the P450 family participate in the 20-E biosynthesis route, and that plants cultured under photoautotrophic conditions promote an upregulated Phantom gene and enhance the productivity of 20-E. The data will be used for future investigations of the 20-E synthesis pathway in P. glomerata while offering a better understanding of the metabolism of the species. |
Palavras-chave: | Brazilian ginseng Pfaffia glomerata Phantom gene Photoautotrophy Phytoecdysteroids Transcriptome |
Editor: | Protoplasma |
Tipo de Acesso: | Springer-Verlag GmbH Austria, part of Springer Nature |
URI: | https://doi.org/10.1007/s00709-018-1322-1 http://www.locus.ufv.br/handle/123456789/24375 |
Data do documento: | 2019 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 2,49 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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