On the cytokine/chemokine network during Plasmodium vivax malaria: new insights to understand the disease

Show simple item record Mendes, Tiago Antônio de Oliveira Hojo-Souza, Natália Satchiko Pereira, Dhelio Batista Souza, Fernanda Sumika Hojo de Cardoso, Mariana Santos Tada, Mauro Shugiro Zanini, Graziela Maria Bartholomeu, Daniella Castanheira Fujiwara, icardo Toshio Bueno, Lilian Lacerda 2017-10-26T16:00:54Z 2017-10-26T16:00:54Z 2017-02-24
dc.identifier.issn 1475-2875
dc.description.abstract The clinical outcome of malaria depends on the delicate balance between pro-inflammatory and immunomodulatory cytokine responses triggered during infection. Despite the numerous reports on characterization of plasma levels of cytokines/chemokines, there is no consensus on the profile of these mediators during blood stage malaria. The identification of acute phase biomarkers might contribute to a better understanding of the disease, allowing the use of more effective therapeutic approaches to prevent the progression towards severe disease. In the present study, the plasma levels of cytokines and chemokines and their association with parasitaemia and number of previous malaria episodes were evaluated in Plasmodium vivax-infected patients during acute and convalescence phase, as well as in healthy donors. Samples of plasma were obtained from peripheral blood samples from four different groups: P. vivax-infected, P. vivax-treated, endemic control and malaria-naïve control. The cytokine (IL-6, IL-10, IL-17, IL-27, TGF-β, IFN-γ and TNF) and chemokine (MCP-1/CCL2, IP-10/CXCL10 and RANTES/CCL5) plasma levels were measured by CBA or ELISA. The network analysis was performed using Spearman correlation coefficient. Plasmodium vivax infection induced a pro-inflammatory response driven by IL-6 and IL-17 associated with an immunomodulatory profile mediated by IL-10 and TGF-β. In addition, a reduction was observed of IFN-γ plasma levels in P. vivax group. A lower level of IL-27 was observed in endemic control group in comparison to malaria-naïve control group. No significant results were found for IL-12p40 and TNF. It was also observed that P. vivax infection promoted higher levels of MCP-1/CCL2 and IP-10/CXCL10 and lower levels of RANTES/CCL5. The plasma level of IL-10 was elevated in patients with high parasitaemia and with more than five previous malaria episodes. Furthermore, association profile between cytokine and chemokine levels were observed by correlation network analysis indicating signature patterns associated with different parasitaemia levels. The P. vivax infection triggers a balanced immune response mediated by IL-6 and MCP-1/CCL2, which is modulated by IL-10. In addition, the results indicated that IL-10 plasma levels are influenced by parasitaemia and number of previous malaria episodes. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Malaria Journal pt-BR
dc.relation.ispartofseries 16:42, January 2017 pt-BR
dc.rights Open Access pt-BR
dc.subject Plasmodium vivax pt-BR
dc.subject Malaria pt-BR
dc.subject Cytokines pt-BR
dc.subject Chemokines pt-BR
dc.title On the cytokine/chemokine network during Plasmodium vivax malaria: new insights to understand the disease en
dc.type Artigo pt-BR

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    Artigos Técnico-científicos nas áreas de Bioquímica e Biologia Molecular

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