Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/12489
Tipo: Artigo
Título: Morphology and contractility in cardiomyocytes of rats with low exercise performance
Autor(es): Quintão Júnior, Judson Fonseca
Natali, Antônio José
Carneiro Júnior, Miguel Araújo
Castro, Cynthia Aparecida de
Drummond, Lucas Rios
Lavorato, Victor Neiva
Felix, Leonardo Bonato
Cruz, Jader dos Santos
Prímola-Gomes, Thales Nicolau
Abstract: Aerobic capacity is essential to physical performance, and low aerobic capacity is related to the triggering of various cardiovascular diseases. To compare the morphology and contractility of isolated rat cardiomyocytes with low performance and standard performance for exercise. Wistar rats with 10 weeks of age underwent a protocol of treadmill running to fatigue, and were divided into two groups: Low Performance (LP) and Standard Performance (SP). Then, the animals were sacrificed, the heart was quickly removed and, by means of enzymatic dissociation, left ventricular cardiomyocytes were isolated. The cell and sarcomeres length and width of cardiomyocytes were measured using an edge detection system. The isolated cardiomyocytes were electrically stimulated at 1 and 3 Hz and cell contraction was measured by registering the change of their length. The cell length was shorter in the LP group (157.2 1.3µm; p < 0.05) compared to SP (161.4 1.3µm), and the same result was observed for the volume of cardiomyocytes (LP, 25.5 0.4. vs. SP, 26.8 ± 0.4 pL; p < 0.05). The time to peak contraction (LP, 116 1 vs. SP 111 2ms) and total relaxation (LP, 143 3 vs. SP 232 3ms) were higher in the LP group. We conclude that left ventricular myocytes of animals with low performance for exercise are smaller than animals with standard performance. In addition to that, they present losses in contractile capacity.
Palavras-chave: Myocytes
Cardiac
Rats
Exercise
Fatigue
Myocardial contraction
Physical conditioning animal
Editor: Arquivos Brasileiros de Cardiologia
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1590/S0066-782X2012005000036
http://www.locus.ufv.br/handle/123456789/12489
Data do documento: 24-Jan-2012
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