Morphology and contractility in cardiomyocytes of rats with low exercise performance

Show simple item record Quintão Júnior, Judson Fonseca Natali, Antônio José Carneiro Júnior, Miguel Araújo Castro, Cynthia Aparecida de Drummond, Lucas Rios Lavorato, Victor Neiva Felix, Leonardo Bonato Cruz, Jader dos Santos Prímola-Gomes, Thales Nicolau 2017-10-30T11:00:21Z 2017-10-30T11:00:21Z 2012-01-24
dc.identifier.issn 0066-782X
dc.description.abstract Aerobic capacity is essential to physical performance, and low aerobic capacity is related to the triggering of various cardiovascular diseases. To compare the morphology and contractility of isolated rat cardiomyocytes with low performance and standard performance for exercise. Wistar rats with 10 weeks of age underwent a protocol of treadmill running to fatigue, and were divided into two groups: Low Performance (LP) and Standard Performance (SP). Then, the animals were sacrificed, the heart was quickly removed and, by means of enzymatic dissociation, left ventricular cardiomyocytes were isolated. The cell and sarcomeres length and width of cardiomyocytes were measured using an edge detection system. The isolated cardiomyocytes were electrically stimulated at 1 and 3 Hz and cell contraction was measured by registering the change of their length. The cell length was shorter in the LP group (157.2 1.3µm; p < 0.05) compared to SP (161.4 1.3µm), and the same result was observed for the volume of cardiomyocytes (LP, 25.5 0.4. vs. SP, 26.8 ± 0.4 pL; p < 0.05). The time to peak contraction (LP, 116 1 vs. SP 111 2ms) and total relaxation (LP, 143 3 vs. SP 232 3ms) were higher in the LP group. We conclude that left ventricular myocytes of animals with low performance for exercise are smaller than animals with standard performance. In addition to that, they present losses in contractile capacity. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Arquivos Brasileiros de Cardiologia pt-BR
dc.relation.ispartofseries vol.98, n.5, p.431-436, Apr. 2012 pt-BR
dc.rights Open Access pt-BR
dc.subject Myocytes pt-BR
dc.subject Cardiac pt-BR
dc.subject Rats pt-BR
dc.subject Exercise pt-BR
dc.subject Fatigue pt-BR
dc.subject Myocardial contraction pt-BR
dc.subject Physical conditioning animal pt-BR
dc.title Morphology and contractility in cardiomyocytes of rats with low exercise performance en
dc.type Artigo pt-BR

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