Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/12821
Tipo: Artigo
Título: Cytogenetic analyses using C-banding and DAPI/CMA3 staining of four populations of the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera, Curculionidae)
Autor(es): Silva, Alexandra A. da
Braga, Lucas S.
Guedes, Raul Narciso C.
Tavares, Mara G.
Abstract: Cytogenetic data avalaible for the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae), one of the most destructive pests of stored cereal grains, are controversial. Earlier studies focused on single populations and emphasized chromosome number and sex determination system. In this paper, the karyotypes of four populations of S. zeamais were characterized by conventional staining, C-banding and sequential staining with the fluorochromes chromomycin-A3/4-6-diamidino-2-phenylindole (CMA3/DAPI). The analyses of metaphases obtained from the cerebral ganglia of last instar larvae and the testes of adults showed that the species had 2n = 22 chromosomes, with 10 autosomal pairs and a sex chromosome pair (XX in females and Xyp in males). Chromosome number, however, ranged from 2n = 22 to 26 due to the presence of 0–4 supernumerary chromosomes in individuals from the populations of Viçosa, Unai and Porto Alegre. With the exception of the Y chromosome, which was dot-like, all other chromosomes of this species were metacentric, including the supernumeraries. The heterochromatin was present in the centromeric regions of all autosomes and in the centromere of the X chromosome. The B chromosomes were partially or totally heterochromatic, and the Y chromosome was euchromatic. The heterochromatic regions were labeled with C-banding and DAPI, which showed that they were rich in AT base pairs.
Palavras-chave: Heterochromatin
B chromosomes
C-banding
Karyotype
Fluorochromes
Editor: Comparative Cytogenetics
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.3897/CompCytogen.v9i1.4611
http://www.locus.ufv.br/handle/123456789/12821
Data do documento: 31-Mar-2015
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