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https://locus.ufv.br//handle/123456789/13890
Tipo: | Artigo |
Título: | Impala, a transposon from Fusarium oxysporum, is active in the genome of Penicillium griseoroseum |
Autor(es): | Queiroz, Marisa Vieira de Daboussi, Marie-Josée |
Abstract: | An autonomous impala transposon trapped in Fusarium oxysporum by insertion within the niaD gene encoding nitrate reductase was introduced in the genome of the fungus Penicillium griseoroseum, a producer of pectinase enzymes. Through a phenotypic assay, we demonstrate that this element is able to excise from the niaD gene and to reinsert at new genomic positions. As in the original host, impala inserts into a TA site and footprints left by impala excisions are generally 5 bp. The fact that impala is able to transpose in P. griseoroseum offers the opportunity to develop a gene-tagging system based on this element with the objective to detect and clone genes related in pectinase production. |
Palavras-chave: | Transposon impala Heterologous transposition Filamentous fungus Penicillium griseoroseum |
Editor: | FEMS Microbiology Letters |
Tipo de Acesso: | Open Access |
URI: | https://doi.org/10.1111/j.1574-6968.2003.tb11535.x http://www.locus.ufv.br/handle/123456789/13890 |
Data do documento: | 12-Dez-2002 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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Queiroz_et_al-2003-FEMS_Microbiology_Letters.pdf | Texto completo | 215,67 kB | Adobe PDF | Visualizar/Abrir |
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