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Impala, a transposon from Fusarium oxysporum, is active in the genome of Penicillium griseoroseum

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dc.contributor.author Queiroz, Marisa Vieira de
dc.contributor.author Daboussi, Marie-Josée
dc.date.accessioned 2017-11-28T13:43:02Z
dc.date.available 2017-11-28T13:43:02Z
dc.date.issued 2002-12-12
dc.identifier.issn 15746968
dc.identifier.uri https://doi.org/10.1111/j.1574-6968.2003.tb11535.x
dc.identifier.uri http://www.locus.ufv.br/handle/123456789/13890
dc.description.abstract An autonomous impala transposon trapped in Fusarium oxysporum by insertion within the niaD gene encoding nitrate reductase was introduced in the genome of the fungus Penicillium griseoroseum, a producer of pectinase enzymes. Through a phenotypic assay, we demonstrate that this element is able to excise from the niaD gene and to reinsert at new genomic positions. As in the original host, impala inserts into a TA site and footprints left by impala excisions are generally 5 bp. The fact that impala is able to transpose in P. griseoroseum offers the opportunity to develop a gene-tagging system based on this element with the objective to detect and clone genes related in pectinase production. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher FEMS Microbiology Letters pt-BR
dc.relation.ispartofseries Volume 218, Issue 2, Pages 317–321, January 2003 pt-BR
dc.rights Open Access pt-BR
dc.subject Transposon impala pt-BR
dc.subject Heterologous transposition pt-BR
dc.subject Filamentous fungus pt-BR
dc.subject Penicillium griseoroseum pt-BR
dc.title Impala, a transposon from Fusarium oxysporum, is active in the genome of Penicillium griseoroseum en
dc.type Artigo pt-BR


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  • Artigos [268]
    Artigos Técnico-científicos na área de Microbiologia

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