Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/14486
Tipo: Artigo
Título: Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders
Autor(es): Pugliesi, Guilherme
Carvalho, Giovanni Ribeiro de
Rates, Daniel Macêdo
Ker, Pedro Gama
Matta, Manuela Pereira da
Oliveira, Renan Reis de
Silva Filho, José Monteiro da
Abstract: Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender) or a glycine egg yolk-based extender (Foote extender) and cooled at 6-8 ºC for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23 ejaculates from two stallions were split-diluted with Kenney extender and Foote extender. The sperm concentration in each extender was adjusted to 500 million viable sperms per insemination dose. Semen was cooled to 6-8 ºC and stored for 24 hours. Seventy-four cycles of crossbred mares were inseminated with either semen diluted in Kenney extender or semen diluted in Foote extender. The pregnancy rate was higher from semen diluted in Kenney extender than that from semen in Foote extender (0.553 vs. 0.306). The Kenney extender is effective in preserving the motility, vigor and fertility of stallion semen after 24 hours of cold storage, whereas the Foote extender is not acceptable.
Palavras-chave: Artificial insemination
Cooling
Semen extender
Stallion
Editor: Revista Brasileira de Zootecnia
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1590/S1516-35982012001200005
http://www.locus.ufv.br/handle/123456789/14486
Data do documento: 3-Ago-2012
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