Use este identificador para citar ou linkar para este item:
Tipo: Artigo
Título: Doxorubicin hinders DNA condensation promoted by the protein bovine serum albumin (BSA)
Autor(es): Lima, C. H. M.
Paula, H. M. C. de
Silva, L. H. M. da
Rocha, M. S.
Abstract: In this work, we have studied the interaction between the anticancer drug doxorubicin (doxo) and condensed DNA, using optical tweezers. To perform this task, we use the protein bovine serum albumin (BSA) in the working buffer to mimic two key conditions present in the real intracellular environment: the condensed state of the DNA and the abundant presence of charged macromolecules in the surrounding medium. In particular, we have found that, when doxo is previously intercalated in disperse DNA, the drug hinders the DNA condensation process upon the addition of BSA in the buffer. On the other hand, when bare DNA is firstly condensed by BSA, doxo is capable to intercalate and to unfold the DNA condensates at relatively high concentrations. In addition, a specific interaction between BSA and doxo was verified, which significantly changes the chemical equilibrium of the DNA–doxo interaction. Finally, the presence of BSA in the buffer stabilizes the double-helix structure of the DNA–doxo complexes, preventing partial DNA denaturation induced by the stretching forces.
Palavras-chave: Bovine serum albumin (BSA)
DNA condensation
Editor: Biopolymers
Tipo de Acesso: Open Access
Data do documento: 23-Out-2017
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
artigo.pdftexto completo1 MBAdobe PDFThumbnail

Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.