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Adaptation of image cytometry methodology for DNA ploidy analysis of cervical epithelium samples: A pilot study

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dc.contributor.author Duarte, Christiane Eliza Motta
dc.contributor.author Carvalho, Carlos Roberto
dc.contributor.author Silva-Filho, Agnaldo Lopes da
dc.date.accessioned 2018-02-21T13:08:39Z
dc.date.available 2018-02-21T13:08:39Z
dc.date.issued 2014-02-05
dc.identifier.issn 1875-6263
dc.identifier.uri http://dx.doi.org/10.1016/j.tjog.2014.04.019
dc.identifier.uri http://www.locus.ufv.br/handle/123456789/17756
dc.description.abstract To determine DNA ploidy in the cervical specimens of patients revealing a suspicion of cancer by image analysis performed by using a combination of commercial analysis software, conventional microscopy, and certified filters. This study followed a prospective design. Cervical samples were obtained from 20 patients undergoing routine screening in the Gynecologic-Oncology Unit of the University Hospital of the Federal University of Minas Gerais, Brazil. Three slides were prepared for each case and the DNA content was determined by image cytometry, post Feulgen staining. DNA ploidy, as well as events exceeding 5C and 9C, was assessed according to the guidelines and algorithms prescribed for diagnostic interpretation by the European Society for Analytical Cellular Pathology. By employing the adapted tool, identification of the lesions with euploid and aneuploid profiles was possible. Abnormal DNA content was found in 65% of the cases (13/20), with 45% (9/20) presenting nuclei with >5C content and 20% (4/20) with >9C content. In the analyses conducted in this study, the coefficient of variation with respect to DNA quantity was lower than the 5% threshold recommended by the European Society for Analytical Cellular Pathology. Image cytometry of the cervical specimens revealed DNA aneuploidy, most probably resulting from chromosomal alterations and appearing as precancerous lesions in 65% of the cases. The adaptations implemented in this study, enabled the DNA-image cytometry to become more accessible, enhancing its extended use as an adjuvant strategy for the early screening of the cervical epithelium samples during routine analyses. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Taiwanese Journal of Obstetrics & Gynecology pt-BR
dc.relation.ispartofseries v. 53, Issue 2, p. 227-231, June 2014 pt-BR
dc.rights Open Access pt-BR
dc.subject Aneuploidy pt-BR
dc.subject Cervical cancer pt-BR
dc.subject Early cancer detection pt-BR
dc.subject Image cytometry pt-BR
dc.title Adaptation of image cytometry methodology for DNA ploidy analysis of cervical epithelium samples: A pilot study en
dc.type Artigo pt-BR


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  • Artigos [703]
    Artigos Técnico-científicos na área de Biologia Geral

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