Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/17970
Tipo: Artigo
Título: Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants
Autor(es): Picolotto, Dayana Rotili Nunes
Barros, Fábio de
Padilha, Daly Roxana Castro
Cruz, Ana Cláudia Ferreira da
Otoni, Wagner Campos
Paiva Neto, Vespasiano Borges de
Abstract: An efficient protocol for in vitro plant propagation of Cyrtopodium paludicolum has been developed using root tips dissected from well-developed seedlings. Root tips were cultured on Knudson medium supplemented with α-naphthaleneacetic acid (NAA), and/or thidiazuron (TDZ). TDZ did not induce protocorm-like bodies (PLBs) in the NAA absence, indicating phytoregulators synergistic effect. Medium supplemented with 1.34 μM NAA and 2.27 μM TDZ resulted in better response on PBLs, and subsequent shoot differentiation (55.25 shoots per explant), and in better rooting number and root length responses, favoring acclimatization with 90% of survived plants. However, the medium supplemented with only NAA (1.34 μM) resulted in 33.50 shoots per explant. Histological sections confirmed that only one PLB was induced per responsive root tip, and it showed numerous dispersed and extended meristemoids, or division centers that originated new PBLs. Additionally, this protocol could be an excellent model to study molecular aspects of root to shoot conversion.
Palavras-chave: Protocorm-like bodies
Thidiazuron
α-naphthaleneacetic acid
Germination
Editor: Crop Breeding and Applied Biotechnology
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1590/1984-70332017v17n3a30
http://www.locus.ufv.br/handle/123456789/17970
Data do documento: 14-Out-2016
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