Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/18409
Tipo: Artigo
Título: Inhibition of herpes simplex virus 1 (HSV-1) and poliovirus (PV-1) by bacteriocins from lactococcus lactis subsp. lactis and enterococcus durans strains isolated from goat milk
Autor(es): Cavicchioli, Valéria Quintana
Carvalho, Otávio Valério de
Paiva, Janine Cerqueira de
Todorov, Svetoslav Dimitrov
Silva Júnior, Abelardo
Nero, Luís Augusto
Abstract: Bacteriocins have unusual inhibitory activity, including antiviral properties, and this can be exploited to give alternative applications. Semi–purified bacteriocins of six lactic acid bacteria (LAB) strains isolated from goat milk (two Lactococcus lactis: GLc03 and GLc05, and four Enterococcus durans: GEn09, GEn12, GEn14 and GEn17) were tested for cytotoxicity in Vero cells (50% Cytotoxicity Concentration: CC50), and for their antiviral activities against herpes simplex virus 1 (HVS-1) and poliovirus (PV-1). Semi-purified bacteriocins presented low cytotoxicity, with CC50 varying from 256.2 µg/mL (GLc05) to 1084.5 µg/mL (GEn14). CC10 was determined for all isolates (GLc03: 36.9 µg/mL; GLc05: 51.2 µg/mL; GEn09: 88.1 µg/mL; GEn12: 99.9 µg/mL; GEn14: 275 µg/mL; and GEn17: 62.2 µg/mL) and considered for antiviral activity assays. Antiviral activity before virus adsorption was recorded against PV-1 for GLc05 (4.9%), GEn09 (3.4%), GEn12 (24.7%) and GEn17 (23.5%), and against HSV-1 for GEn12 (27.9%), GEn14 (58.7%) and GEn17 (39.2%). Antiviral activity after virus adsorption was identified against PV-1 for GLc05 (32.7%), GEn09 (91.0%), GEn12 (93.7%) and GEn17 (57.2%), and against HSV-1 for GEn17 (71.6%). The results obtained indicate the potential of some bacteriocins, particularly those produced by E. durans strains investigated in the present study, in viral inhibition and their application as new antiviral agents.
Palavras-chave: Lactic acid bacteria
Bacteriocins
Antiviral activity
Herpes virus
Poliovirus
Editor: International Journal of Antimicrobial Agents
Tipo de Acesso: Elsevier B.V. and the International Society of Chemotherapy
URI: https://doi.org/10.1016/j.ijantimicag.2017.04.020
http://www.locus.ufv.br/handle/123456789/18409
Data do documento: 5-Abr-2017
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