Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/18512
Tipo: Artigo
Título: Morphological and molecular differences in corpus luteum of pregnant sows from divergent genetic groups
Autor(es): Costa, Karine A.
Silva, Walmir da
Veroneze, Renata
Montes, José C.
Verardo, Lucas L.
Botelho, Margareth E.
Duarte, Marcio S.
Neves, Mariana M.
Lopes, Paulo S.
Guimarães, José D.
Teixeira, Susana A.
Alcantara, Laene
Guimarães, Simone E.F.
Abstract: Comprehending mechanisms controlling corpus luteum (CL) angiogenesis and apoptosis in pregnant sows is essential to understand the physiological role of these processes in CL function, progesterone production and consequently in conceptus development and prenatal mortality. CL from 54 sows from two genetic groups, a commercial line (COM) and the local Piau breed (LPB), were obtained for gene expression (n = 3 COM; n = 6 LPB), histological and protein analysis (n = 3 COM; n = 3 LPB), divided in six gestational ages (seven, 15, 30, 45, 60 and 90 days). We observed differences between gestational ages in CL morphology, in which the average number of blood vessels/capillaries at 90-days was greater than at the seventh day by Tukey test. RT-qPCR analysis revealed that apoptotic genes (BAX, BCL2 and CASP3) were differentially expressed between genetic groups and gestational ages in each group. Angiogenesis genes also presented differences between genetic groups (ANGPT1) and gestational ages (MMP9, VEGFA and ANGPT1). No differences in protein abundance of steroidogenic enzymes (CYP11A1 and HSD3B1) were observed. Our findings indicate that despite the differences in gene expression, differences in corpus luteum vascularization were observed only across gestational ages, with no dissimilarities between genetic groups.
Palavras-chave: Angiogenesis
Apoptosis
Gene expression
Gestation
Editor: Theriogenology
Tipo de Acesso: Elsevier Inc. All rights reserved.
URI: https://doi.org/10.1016/j.theriogenology.2017.07.048
http://www.locus.ufv.br/handle/123456789/18512
Data do documento: 29-Jul-2017
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