Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/18791
Tipo: Artigo
Título: Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects
Autor(es): Cruz, Ana Cláudia F.
Cipriano, Jamile L.D.
Mancini, Karina C.
Schmildt, Edilson R.
Lopes, José Carlos
Otoni, Wagner C.
Alexandre, Rodrigo S.
Abstract: The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heart-shape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L^-1) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus^-1) and the lowest percentage of early germination (4%).
Palavras-chave: Carica papaya L.
Juvenile explants
Maturation
Microscopic analysis
Synthetic auxins
Editor: Anais da Academia Brasileira de Ciências
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1590/0001-3765201820160252
http://www.locus.ufv.br/handle/123456789/18791
Data do documento: 1-Fev-2018
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