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Tipo: Artigo
Título: Applying functional metagenomics to search for novel lignocellulosic enzymes in a microbial consortium derived from a thermophilic composting phase of sugarcane bagasse and cow manure
Autor(es): Colombo, Lívia Tavares
Oliveira, Marcelo Nagem Valério de
Carneiro, Deisy Guimarães
Souza, Robson Assis de
Alvim, Mariana Caroline Tocantins
Santos, Josenilda Carlos dos
Silva, Cynthia Canêdo da
Vidigal, Pedro Marcus Pereira
Silveira, Wendel Batista da
Passos, Flávia Maria Lopes
Abstract: Environments where lignocellulosic biomass is naturally decomposed are sources for discovery of new hydrolytic enzymes that can reduce the high cost of enzymatic cocktails for second-generation ethanol production. Metagenomic analysis was applied to discover genes coding carbohydrate-depleting enzymes from a microbial laboratory subculture using a mix of sugarcane bagasse and cow manure in the thermophilic composting phase. From a fosmid library, 182 clones had the ability to hydrolyse carbohydrate. Sequencing of 30 fosmids resulted in 12 contigs encoding 34 putative carbohydrate-active enzymes belonging to 17 glycosyl hydrolase (GH) families. One third of the putative proteins belong to the GH3 family, which includes β-glucosidase enzymes known to be important in the cellulose-deconstruction process but present with low activity in commercial enzyme preparations. Phylogenetic analysis of the amino acid sequences of seven selected proteins, including three β-glucosidases, showed low relatedness with protein sequences deposited in databases. These findings highlight microbial consortia obtained from a mixture of decomposing biomass residues, such as sugar cane bagasse and cow manure, as a rich resource of novel enzymes potentially useful in biotechnology for saccharification of lignocellulosic substrate.
Palavras-chave: Cellulases genes
Cellulose degradation
Fosmid library sequencing
Metagenomic library
Editor: Antonie van Leeuwenhoek
Tipo de Acesso: Springer International Publishing Switzerland
Data do documento: 27-Jun-2016
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  Until 2100-12-31
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