Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/18870
Tipo: Artigo
Título: In vitro genotoxicity of Melaleuca alternifolia essential oil in human lymphocytes
Autor(es): Pinheiro, Antônio Lelis
Pereira, Tais Susane
Sant'Anna, Juliane Rocha de
Silva, Expedito Leite
Castro-Prado, Marialba A.Alves de
Abstract: The volatile essential oil derived from the plant Melaleuca alternifolia, also called tea tree oil (TTO), is largely employed for its antimicrobial properties against several human pathogens. It is used in many topical formulations to treat cutaneous infections.Since very few studies have been done on the safety and toxicity of the crude Melaleuca alternifolia essential oil, current investigation evaluates the possible genotoxic effects of TTO in human lymphocyte cultures.The composition of current TTO sample was determined by GC/MS and NMR. The level of cytotoxicity in TTO treated cultures was determined by decrease of mitotic index when compared to that in negative control. The genotoxic potential of TTO was assessed by the in vitro mammalian cell micronucleus and the chromosome aberrations (CA) tests.Twenty-seven compounds were identified, accounting for 98.9% of the constituents. Terpinen-4-ol (42.8%), γ-terpinene (20.4%), p-cymene (9.6%), α-terpinene (7.9%), 1,8-cineole (3%), α-terpineol (2.8%) and α-pinene (2.4%) were the major compounds of the oil sample. None of the tested TTO concentrations (95 μg/ml, 182 μg/ml and 365 μg/ml) caused a significant increase in the observed frequencies of micronuclei when compared to those in the untreated cultures (negative control). Additionally, no significant differences regarding the frequencies of CA were observed among the tested TTO concentrations and the negative control.Results demonstrate that TTO, in the tested concentrations, is not genotoxic in in vitro mammalian cells.
Palavras-chave: Tea tree oil
Melaleuca alternifolia
Genotoxicity
In vitro mammalian cell micronucleus
Chromosomal aberration test
Mitotic index
Editor: Journal of Ethnopharmacology
Tipo de Acesso: Elsevier Ireland Ltd
URI: https://doi.org/10.1016/j.jep.2013.11.045
http://www.locus.ufv.br/handle/123456789/18870
Data do documento: 3-Fev-2014
Aparece nas coleções:Engenharia Florestal - Artigos

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