Acyl homoserine lactone-based quorum sensing stimulates biofilm formation by Salmonella Enteritidis in anaerobic conditions

Show simple item record Almeida, Felipe Alves de Pimentel-Filho, Natan de Jesus Pinto, Uelinton Manoel Mantovani, Hilário Cuquetto Oliveira, Leandro Licursi de Vanetti, Maria Cristina Dantas 2018-04-19T19:58:13Z 2018-04-19T19:58:13Z 2016-11-12
dc.identifier.issn 1432072X
dc.description.abstract Quorum sensing regulates a variety of phenotypes in bacteria including the production of virulence factors. Salmonella spp. have quorum sensing systems mediated by three autoinducers (AI-1, AI-2, and AI-3). The AI-1-mediated system is incomplete in that the bacterium relies on the synthesis of signaling molecules by other microorganisms. This study aimed to evaluate the influence of the AI-1 N-dodecanoyl-DL-homoserine lactone (C12-HSL) on the growth, motility, adhesion, and biofilm formation of Salmonella enterica serovar Enteritidis PT4 578 on a polystyrene surface. Experiments were conducted at 37 °C in anaerobic tryptone soy broth supplemented with C12-HSL and/or a mixture of four synthetic furanones, at the concentration of 50 nM each. The planktonic growth, adhesion, swarming, and twitching motility were not altered in the presence of C12-HSL and/or furanones under anaerobic conditions. However, C12-HSL induced biofilm formation after 36 h of cultivation as determined by quantification of biofilm formation, by enumeration of adhered cells to polystyrene coupons, and finally by imaging the presence of multilayered cells on an epifluorescence microscope. When furanones were present in the medium, an antagonistic effect against C12-HSL on the biofilm development was observed. The results demonstrate an induction of biofilm formation in Salmonella Enteritidis by AI-1 under anaerobic conditions. Considering that Salmonella does not produce AI-1 but respond to it, C12-HSL synthesized by other bacterial species could trigger biofilm formation by this pathogen in conditions that are relevant for its pathogenesis. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Archives of Microbiology pt-BR
dc.relation.ispartofseries v. 199, Issue 3, p. 475–486, April 2017 pt-BR
dc.rights Springer-Verlag Berlin Heidelberg pt-BR
dc.subject Adhesion pt-BR
dc.subject Autoinducer pt-BR
dc.subject Biofilm pt-BR
dc.subject Cellular communication pt-BR
dc.subject Furanone pt-BR
dc.title Acyl homoserine lactone-based quorum sensing stimulates biofilm formation by Salmonella Enteritidis in anaerobic conditions en
dc.type Artigo pt-BR

Files in this item

This item appears in the following Collection(s)

  • Artigos [265]
    Artigos Técnico-científicos na área de Microbiologia

Show simple item record

Search DSpace


My Account