Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/18947
Tipo: Artigo
Título: Mesenchymal stem cell therapy associated with endurance exercise training: Effects on the structural and functional remodeling of infarcted rat hearts
Autor(es): Lavorato, Victor Neiva
Carlo, Ricardo Junqueira Del
Cunha, Daise Nunes Queiroz da
Okano, Barbara Silva
Belfort, Felipe Gomes
Freitas, Juliana Silveira de
Mota, Gloria de Fatima Alves da
Quintão-Júnior, Judson Fonseca
Silame-Gomes, Luis Henrique Lobo
Drummond, Filipe Rios
Carneiro-Júnior, Miguel Araújo
Oliveira, Edilamar Menezes de
Monteiro, Betania Souza
Prímola-Gomes, Thales Nicolau
Natali, Antônio José
Abstract: We tested the effects of early mesenchymal stem cell (MSC) therapy associated with endurance exercise on the structural and functional cardiac remodeling of rats with myocardial infarctation (MI). Male Wistar rats (40 days old) were divided into 6 groups: control and exercise sham; control and exercise MI; and control and exercise MI MSC. MI was surgically induced and bone marrow-derived MSCs were immediately injected via caudal vein (concentration: 1 × 106 cells). Twenty-four hours later ET groups exercised on a treadmill (5 days/week; 60 min/day; 60% of maximal running velocity) for 12 weeks. Structural and functional changes were determined by echocardiography. Contractility and intracellular global calcium ([Ca2 +]i) transient were measured in myocytes from the left ventricular (LV) non-infarcted area. Calcium regulatory proteins were measured by Western blot. MI increased (p < 0.05) heart, ventricular and LV weights and its ratios to body weight; LV internal dimension in diastole (LVID-D) and in systole (LVID-S) and LV free wall in diastole (LVFW-D), but reduced the thickness of interventricular septum in systole (IVS-S), ejection fraction (EF) and fractional shortening (FS). MI augmented (p < 0.05) the times to peak and to half relaxation of cell shortening as well as the amplitude of the [Ca2 +]i transient and the times to peak and to half decay. Early MSCs therapy restored LVFW-D, IVS-S and the amplitude and time to half decay of the [Ca2 +]i transient. Early endurance exercise intervention increased (p < 0.05) LVFW-S, IVS-S, EF and FS, and reduced the times to peak and to half relaxation of cell shortening, and the amplitude of the [Ca2 +]i transient. Exercise training also increased the expression of left ventricular SERCA2a and PLBser16. Nevertheless, the combination of these therapies did not cause additive effects. In conclusion, combining early MSCs therapy and endurance exercise does not potentiate the benefits of such treatments to structural and functional cardiac remodeling in infarcted rats
Palavras-chave: Cardiomyocytes
Stem cells
Endurance training
Myocardial infarction
Editor: Journal of Molecular and Cellular Cardiology
Tipo de Acesso: Elsevier Ltd.
URI: https://doi.org/10.1016/j.yjmcc.2015.12.012
http://www.locus.ufv.br/handle/123456789/18947
Data do documento: 15-Dez-2015
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