Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19090
Tipo: Artigo
Título: Ocelot and oncilla spermatozoa can bind hen egg perivitelline membranes
Autor(es): Araujo, Gediendson Ribeiro de
Paula, Tarcizio Antônio Rego de
Deco-Souza, Thyara de
Garay, Rafael de Morais
Bergo, C.F. Letícia
Csermak-Júnior, Antônio Carlos
Silva, Leanes Cruz da
Alves, Saullo Vinícius Pereira
Abstract: We evaluated the capacity of ocelot and oncilla spermatozoa to bind to the perivitelline membranes (PVMs) of hen eggs in a sperm binding assay (S-PVM). In addition, a device that improves the standardization of the assay was developed. The number of sperm bound to the PVM in fresh (T1) and frozen–thawed (T2) semen from both species was compared to the sperm quality observed in routine tests. The PVM was stretched on a circular silicone device to create a standardized area for analysis. In both treatments and for both species, the spermatozoa were able to bind to the PVM, indicating that PVM may be used for a sperm binding assay in ocelot and oncilla. The S-PVM assay did not differ in fresh and frozen–thawed ocelot sperm (p > 0.05). However, fewer oncilla sperm (p < 0.05) were bound to the PVM in T2, indicating that the proposed test may be able to detect injuries that compromise sperm binding abilities. The device maintained the PVM stretched during the processing and defined the evaluation area.
Palavras-chave: Semen
Leopardus pardalis
Leopardus tigrinus
Editor: Animal Reproduction Science
Tipo de Acesso: Elsevier B.V.
URI: https://doi.org/10.1016/j.anireprosci.2015.09.018
http://www.locus.ufv.br/handle/123456789/19090
Data do documento: 13-Out-2015
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