Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19305
Tipo: Artigo
Título: Yersinia enterocolitica in a Brazilian pork production chain: Tracking of contamination routes, virulence and antimicrobial resistance
Autor(es): Martins, Bruna Torres Furtado
Botelho, Clarisse Vieira
Silva, Danilo Augusto Lopes
Lanna, Frederico Germano Piscitelli Alvarenga
Grossi, Juliana Libero
Campos-Galvão, Maria Emilene Martino
Yamatogi, Ricardo Seiti
Falcão, Juliana Pfrimer
Bersot, Luciano dos Santos
Nero, Luís Augusto
Abstract: This study aimed to track Yersinia enterocolitica contamination in a pork production chain in Minas Gerais, Brazil, and to characterize the virulence and antibiotic resistance of isolates. Samples were collected from four different steps of the pork production chain (pig farm, carcass, processing environment and end product; n = 870), and tested for the presence of Y. enterocolitica. The pathogen was detected in 8 samples (palatine tonsils = 5; mesenteric lymph nodes = 2; carcass after bleeding = 1), from which 16 isolates were obtained and identified as Y. enterocolitica bioserotype 4/O:3. XbaI macrorestriction allowed the clustering of isolates in 5 pulsetypes, and the identification of identical profiles of Y. enterocolitca isolated from different samples. All isolates were positive for the virulence related genes ail, virF, myfA, ystA, tccC, ymoA, hreP and sat, and negative for ystB, ystC, fepA, fepD and fes. Considering 17 antibiotics from 11 classes, only ciprofloxacin and kanamycin were effective against all isolates, and three multidrug resistance profiles were identified among them, with simultaneous resistance to 9 of 11 classes. All isolates presented positive results for emrD, yfhD and marC, related to multidrug resistance. The results of this study demonstrated the contamination routes of Y. enterocolitica within the assessed pork production chain, and highlighted the pathogenic potential and antibiotic resistance of this foodborne pathogen.
Palavras-chave: Yersinia enterocolitica
Pork chain
Pulsed-field gel electrophoresis
Virulence
Antimicrobial resistance
Editor: International Journal of Food Microbiology
Tipo de Acesso: Elsevier B.V.
URI: https://doi.org/10.1016/j.ijfoodmicro.2018.03.028
http://www.locus.ufv.br/handle/123456789/19305
Data do documento: 3-Abr-2018
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