Mobilisation of bacteria in a fine-grained residual soil by electrophoresis

Show simple item record Rocha, Ulisses Nunes Da Tótola, Marcos Rogério Pessoa, Denise Maria Mano Araruna Júnior, José Tavares Neves, Júlio César Lima Borges, Arnaldo Chaer 2018-05-08T11:15:26Z 2018-05-08T11:15:26Z 2009-01-15
dc.identifier.issn 03043894
dc.description.abstract An investigation of electrokinetic bacterial mobilisation in a residual soil from gneiss is presented here. The experimental program aimed at assessing the efficacy of electrophoresis against the electro-osmotic flow to transport endospores of Bacillus subtilis LBBMA 155 and nitrogen-starved cells of Pseudomonas sp. LBBMA 81. Electrokinesis was performed on a low hydraulic reconstituted clayey soil column submitted to a 5 mA electrical current for 24 h. Cells were coccoid-shaped and characterised as possessing low surface hydrophobicity and less than 1 μm in diameter. Distribution coefficient for B. subtilis in the soil was between 16.8 and 19.9 times higher than that for Pseudomonas sp. Distribution coefficient for B. subtilis between eluate and anionic exchange column was 11.8 times higher than that for Pseudomonas sp. After the electrokinesis, it was shown that cells and endospores were distributed hyperbolically through the soil probe and moved against the electro-osmotic flow; however, endospores were transported throughout all soil core and starved cells only till half of its length. The higher transport efficiency of B. subtilis endospores was attributed to their higher negative charge on cell surface. These results demonstrate that electrokinesis can be used for bacteria transport in soils with low hydraulic conductivity, even against the electro-osmotic flow. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Journal of Hazardous Materials pt-BR
dc.relation.ispartofseries v. 161, n. 1, p. 485-491, january 2009 pt-BR
dc.rights Elsevier B.V. pt-BR
dc.subject Electrokinesis pt-BR
dc.subject Bacterial electrophoresis pt-BR
dc.subject Cell transport pt-BR
dc.subject Bacterial starvation pt-BR
dc.title Mobilisation of bacteria in a fine-grained residual soil by electrophoresis en
dc.type Artigo pt-BR

Files in this item

This item appears in the following Collection(s)

  • Artigos [268]
    Artigos Técnico-científicos na área de Microbiologia

Show simple item record

Search DSpace


My Account