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https://locus.ufv.br//handle/123456789/19406
Tipo: | Artigo |
Título: | Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk |
Autor(es): | Cavicchioli, Valéria Quintana Dornellas, Wesley dos Santos Perin, Luana Martins Pieri, Fábio Alessandro Franco, Bernadette Dora Gombossy de Melo Todorov, Svetoslav Dimitrov Nero, Luís Augusto |
Abstract: | Lactic acid bacteria (LAB, n = 57) were previously obtained from raw goat milk, identified as Lactococcus spp. (n = 24) and Enterococcus spp. (n = 33), and characterized as bacteriocinogenic. Fingerprinting by pulsed field gel electrophoresis (PFGE) demonstrated high genetic diversity, and 30 strains were selected and exhibited strong antimicrobial activity against 46 target strains (LAB, spoilage, and foodborne pathogens). Six strains (Lactococcus lactis: GLc03 and GLc05; and Enterococcus durans: GEn09, GEn12, GEn14, and GEn17) were selected to characterize their bacteriocinogenic features, using Listeria monocytogenes ATCC 7644 as the target. The six strains produced bacteriocins at higher titer when incubated in MRS at 37 °C up to 12 h, when compared to growth at 25 and 30 °C. The produced bacteriocins kept their antimicrobial activity after exposure to 100 °C for 2 h and 121 °C for 20 min; the antimicrobial activity was also observed after treatment at pH 2.0 to 10.0, except for GLc03. L. monocytogenes populations were reduced approximately two logs after treatment with cell-free supernatants from the selected strains. These data show that goat milk can contain a diverse microbiota able to inhibit L. monocytogenes, a common pathogen found in dairy products, and can be potentially employed in biopreservation of food produced under different processing conditions. |
Palavras-chave: | Goat milk Lactic acid bacteria Bacteriocins PFGE Inhibition Listeria monocytogenes |
Editor: | Applied Biochemistry and Biotechnology |
Tipo de Acesso: | Springer Science+Business Media New York |
URI: | https://doi.org/10.1007/s12010-015-1511-8 http://www.locus.ufv.br/handle/123456789/19406 |
Data do documento: | 31-Jan-2015 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 475,73 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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