Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19406
Tipo: Artigo
Título: Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk
Autor(es): Cavicchioli, Valéria Quintana
Dornellas, Wesley dos Santos
Perin, Luana Martins
Pieri, Fábio Alessandro
Franco, Bernadette Dora Gombossy de Melo
Todorov, Svetoslav Dimitrov
Nero, Luís Augusto
Abstract: Lactic acid bacteria (LAB, n = 57) were previously obtained from raw goat milk, identified as Lactococcus spp. (n = 24) and Enterococcus spp. (n = 33), and characterized as bacteriocinogenic. Fingerprinting by pulsed field gel electrophoresis (PFGE) demonstrated high genetic diversity, and 30 strains were selected and exhibited strong antimicrobial activity against 46 target strains (LAB, spoilage, and foodborne pathogens). Six strains (Lactococcus lactis: GLc03 and GLc05; and Enterococcus durans: GEn09, GEn12, GEn14, and GEn17) were selected to characterize their bacteriocinogenic features, using Listeria monocytogenes ATCC 7644 as the target. The six strains produced bacteriocins at higher titer when incubated in MRS at 37 °C up to 12 h, when compared to growth at 25 and 30 °C. The produced bacteriocins kept their antimicrobial activity after exposure to 100 °C for 2 h and 121 °C for 20 min; the antimicrobial activity was also observed after treatment at pH 2.0 to 10.0, except for GLc03. L. monocytogenes populations were reduced approximately two logs after treatment with cell-free supernatants from the selected strains. These data show that goat milk can contain a diverse microbiota able to inhibit L. monocytogenes, a common pathogen found in dairy products, and can be potentially employed in biopreservation of food produced under different processing conditions.
Palavras-chave: Goat milk
Lactic acid bacteria
Bacteriocins
PFGE
Inhibition
Listeria monocytogenes
Editor: Applied Biochemistry and Biotechnology
Tipo de Acesso: Springer Science+Business Media New York
URI: https://doi.org/10.1007/s12010-015-1511-8
http://www.locus.ufv.br/handle/123456789/19406
Data do documento: 31-Jan-2015
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