Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19486
Tipo: Artigo
Título: Immunogenomic screening approach to identify new antigens for the serological diagnosis of chronic Chagas’ disease
Autor(es): Elisei, Rutyanne Maria Tonelli
Matos, Christiane Santos
Carvalho, Ana Maria Ravena Severino
Chaves, Ana Thereza
Medeiros, Fernanda Alvarenga Cardoso
Barbosa, Ronaldo
Marcelino, Andreza Pain
Emidio, Kenia dos Santos
Coelho, Eduardo Antonio Ferraz
Duarte, Mariana Costa
Mendes, Tiago Antônio de Oliveira
Rocha, Manoel Otávio da Costa
Menezes-Souza, Daniel
Abstract: Serological tests are preferentially used for the diagnosis of Chagas’ disease (CD) during the chronic phase because of the low parasitemia and high anti-Trypanosoma cruzi antibody titers. However, the current methods showed several disadvantages, as contradictory or inconclusive results, mainly related to the characteristics of the antigens used, in general, crude or whole parasites, but also due to antigen production protocol and the experimental conditions used in serological tests. Thus, better-quality serological assays are urgently needed. Here, we performed a wide immunogenomic screen strategy to identify conserved linear B-cell epitopes in the predicted proteome based on genome sequence from T. cruzi strains to will be applied as synthetic peptides in the serodiagnosis of the chronic CD. Three B-cell epitopes derived from mucin-associated surface protein (MASP) family, expressed in both infective parasite stages, trypomastigote and amastigotes, conserved in T. cruzi strains, and highly divergent as compared with Leishmania spp. proteome, were selected for this study. The results demonstrated that synthetic peptide 2 and a mixture of peptides (Mix II: peptides 2 and 3) were able to identify all chronic CD cases, indeterminate or Chagas cardiomyopathy clinical presentation, and simultaneously able to discriminate infections caused by Leishmania parasites, with high accuracy (98.37 and 100.00%, respectively) and agreement (kappa index = 0.967 and 1.000, respectively) with direct methods as compared to current diagnostic pipeline performed by reference laboratories in Brazil. This study represents an interesting strategy for the discovery of new antigens applied to serologic diagnosis of infectious diseases and for the technological development of platforms for large-scale production of diagnostic tests.
Palavras-chave: Trypanosoma cruzi
Chagas’ disease
Immunoinformatics
B-cell epitope mapping
Immunodiagnosis
Peptides
Editor: Applied Microbiology and Biotechnology
Tipo de Acesso: Springer-Verlag GmbH Germany, part of Springer Nature 2
URI: https://doi.org/10.1007/s00253-018-8992-7
http://www.locus.ufv.br/handle/123456789/19486
Data do documento: 7-Mai-2018
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