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https://locus.ufv.br//handle/123456789/19486
Tipo: | Artigo |
Título: | Immunogenomic screening approach to identify new antigens for the serological diagnosis of chronic Chagas’ disease |
Autor(es): | Elisei, Rutyanne Maria Tonelli Matos, Christiane Santos Carvalho, Ana Maria Ravena Severino Chaves, Ana Thereza Medeiros, Fernanda Alvarenga Cardoso Barbosa, Ronaldo Marcelino, Andreza Pain Emidio, Kenia dos Santos Coelho, Eduardo Antonio Ferraz Duarte, Mariana Costa Mendes, Tiago Antônio de Oliveira Rocha, Manoel Otávio da Costa Menezes-Souza, Daniel |
Abstract: | Serological tests are preferentially used for the diagnosis of Chagas’ disease (CD) during the chronic phase because of the low parasitemia and high anti-Trypanosoma cruzi antibody titers. However, the current methods showed several disadvantages, as contradictory or inconclusive results, mainly related to the characteristics of the antigens used, in general, crude or whole parasites, but also due to antigen production protocol and the experimental conditions used in serological tests. Thus, better-quality serological assays are urgently needed. Here, we performed a wide immunogenomic screen strategy to identify conserved linear B-cell epitopes in the predicted proteome based on genome sequence from T. cruzi strains to will be applied as synthetic peptides in the serodiagnosis of the chronic CD. Three B-cell epitopes derived from mucin-associated surface protein (MASP) family, expressed in both infective parasite stages, trypomastigote and amastigotes, conserved in T. cruzi strains, and highly divergent as compared with Leishmania spp. proteome, were selected for this study. The results demonstrated that synthetic peptide 2 and a mixture of peptides (Mix II: peptides 2 and 3) were able to identify all chronic CD cases, indeterminate or Chagas cardiomyopathy clinical presentation, and simultaneously able to discriminate infections caused by Leishmania parasites, with high accuracy (98.37 and 100.00%, respectively) and agreement (kappa index = 0.967 and 1.000, respectively) with direct methods as compared to current diagnostic pipeline performed by reference laboratories in Brazil. This study represents an interesting strategy for the discovery of new antigens applied to serologic diagnosis of infectious diseases and for the technological development of platforms for large-scale production of diagnostic tests. |
Palavras-chave: | Trypanosoma cruzi Chagas’ disease Immunoinformatics B-cell epitope mapping Immunodiagnosis Peptides |
Editor: | Applied Microbiology and Biotechnology |
Tipo de Acesso: | Springer-Verlag GmbH Germany, part of Springer Nature 2 |
URI: | https://doi.org/10.1007/s00253-018-8992-7 http://www.locus.ufv.br/handle/123456789/19486 |
Data do documento: | 7-Mai-2018 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 1,46 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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