Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19599
Tipo: Artigo
Título: A porcine circovirus-2 mutant isolated in Brazil contains low-frequency substitutions in regions of immunoprotective epitopes in the capsid protein
Autor(es): Salgado, Rafael Locatelli
Vidigal, Pedro Marcus Pereira
Gonzaga, Natalia F.
Souza, Luiz F. L. de
Polêto, Marcelo D.
Onofre, Thiago Souza
Eller, Monique R.
Pereira, Carlos Eduardo Real
Fietto, Juliana L. R.
Bressan, Gustavo C.
Guedes, Roberto M. C.
Almeida, Márcia R.
Silva Júnior, Abelardo
Abstract: Porcine circovirus-2 (PCV2) is the etiologic agent of several diseases in pigs, including multi-systemic wasting syndrome (PMWS). In this work, a new mutant PCV2b was isolated from PMWS-affected pigs on a Brazilian farm. Its genome showed high sequence similarity (>99 % identity) to those from a group of emerging mutants isolated from cases of PMWS outbreaks in vaccinated pigs in China, the USA and South Korea. Here, we show that these isolates share a combination of low-frequency substitutions (single amino acid polymorphisms with a frequency of ≤25 %) in the viral capsid protein, mainly in regions of immunoprotective epitopes, and an additional lysine residue at position 234. These isolates were phylogenetically grouped in the PCV2b clade, reinforcing the idea of the emergence of a new group of mutants PCV2b associated with outbreaks worldwide. The identification of these polymorphisms in the viral capsid highlights the importance of considering these isolates for the development of more-effective vaccines.
Palavras-chave: Capsid Protein
Viral capsid
Conformational Epitope
Viral Capsid Protein
Classical strain
Editor: Archives of Virology
Tipo de Acesso: Springer-Verlag Wien
URI: http://dx.doi.org/10.1007/s00705-015-2567-z
http://www.locus.ufv.br/handle/123456789/19599
Data do documento: 15-Ago-2015
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
artigo.pdf
  Until 2100-12-31
texto completo1,28 MBAdobe PDFVisualizar/Abrir    Solictar uma cópia


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.