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https://locus.ufv.br//handle/123456789/19612
Tipo: | Artigo |
Título: | Measurements of Cry1F binding and activity of luminal gut proteases in susceptible and Cry1F resistant Ostrinia nubilalis larvae (Lepidoptera: Crambidae) |
Autor(es): | Pereira, Eliseu J.G. Siqueira, Herbert A.A. Zhuang, Meibao Storer, Nicholas P. Siegfried, Blair D. |
Abstract: | The biochemical mechanism of resistance to the Bacillus thuringiensis Cry1F toxin was studied in a laboratory-selected strain of Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) showing more than 3000-fold resistance to Cry1F and limited cross resistance to other Cry toxins. Analyses of Cry1F binding to brush border membrane vesicles of midgut epithelia from susceptible and resistant larvae using ligand immunoblotting and Surface Plasmon Resonance (SPR) suggested that reduced binding of Cry1F to insect receptors was not associated with resistance. Additionally, no differences in activity of luminal gut proteases or altered proteolytic processing of the toxin were observed in the resistant strain. Considering these results along with previous evidence of relatively narrow spectrum of cross resistance and monogenic inheritance, the resistance mechanism in this Cry1F selected strain of O. nubilalis appears to be specific and may be distinct from previously identified resistance mechanisms reported in other Lepidoptera. |
Palavras-chave: | Bacillus thuringiensis Bt maize Ostrinia nubilalis Resistance Toxin binding Luminal gut proteases Surface Plasmon Resonance |
Editor: | Journal of Invertebrate Pathology |
Tipo de Acesso: | Elsevier Inc. |
URI: | https://doi.org/10.1016/j.jip.2009.08.014 http://www.locus.ufv.br/handle/123456789/19612 |
Data do documento: | 17-Set-2009 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 582,52 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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