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Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase

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dc.contributor.author Pereira, José Antonio Marques
dc.contributor.author Rosa, Paulo De Tarso Vieira E.
dc.contributor.author Pastore, Glaucia Maria
dc.contributor.author Santana, Cesar Costapinto
dc.date.accessioned 2018-05-16T13:55:59Z
dc.date.available 2018-05-16T13:55:59Z
dc.date.issued 1998-03
dc.identifier.issn 15590291
dc.identifier.uri https://doi.org/10.1007/BF02920188
dc.identifier.uri http://www.locus.ufv.br/handle/123456789/19616
dc.description.abstract The application of expanded-bed ion-exchange resins allows the elimination of intermediary particulate separation steps like filtration or centrifugation prior to adsorption steps in enzyme-purification processes from crude fermentation broths. This work is concerned with the experimental evaluation data of a process related to the adsorption of an extracellular p-galactosidase from the fungiScopulariopsis. The protein recovery in the ion-exchange resin Accell Plus QMA™ was accomplished using a continuous-monitoring method. The direct adsorption step was followed by a elution step with concentrated NaCl solutions aiming to improve the enzyme-specific activity. Experimental data for fixed and expanded bed were compared en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Applied Biochemistry and Biotechnology pt-BR
dc.relation.ispartofseries v. 70, Issue 1, p. 779–787, March 1998 pt-BR
dc.rights Humana Press Inc. pt-BR
dc.subject Extracellular p-galactosidase pt-BR
dc.subject Adsorption pt-BR
dc.subject Expanded bed pt-BR
dc.subject Enzyme recovery pt-BR
dc.title Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase en
dc.type Artigo pt-BR


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  • Artigos [313]
    Artigos Técnico-científicos na área de Tecnologia de Alimentos

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