Locus  

High genetic variability in endophytic fungi from the genus Diaporthe isolated from common bean (Phaseolus vulgaris L.) in Brazil

Show simple item record

dc.contributor.author Santos, T.T. dos
dc.contributor.author Souza Leite, T. de
dc.contributor.author Queiroz, C.B. de
dc.contributor.author Araújo, E.F. de
dc.contributor.author Pereira, O.L.
dc.contributor.author Queiroz, M.V. de
dc.date.accessioned 2018-05-18T11:49:03Z
dc.date.available 2018-05-18T11:49:03Z
dc.date.issued 2015-11-06
dc.identifier.issn 13645072
dc.identifier.uri http://dx.doi.org/10.1111/jam.12985
dc.identifier.uri http://www.locus.ufv.br/handle/123456789/19676
dc.description.abstract The goals of the present study were to identify, to analyse the phylogenetic relations and to evaluate the genetic variability in Diaporthe endophytic isolates from common bean. Diaporthe sp., D. infecunda and D. phaseolorum strains were identified using multilocus phylogeny (rDNA ITS region; EF1‐α, β‐tubulin, and calmodulin genes). IRAP (Inter‐Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon‐Microsatellite Amplified Polymorphism) molecular markers reveal the existence of high genetic variability, especially among D. infecunda isolates. It was concluded that the multilocus phylogenetic approach was more effective than individual analysis of ITS sequences, in identifying the isolates to species level, and that IRAP and REMAP markers can be used for studying the genetic variability in the genus Diaporthe particularly at the intraspecific level. The combined use of molecular tools such as multilocus phylogenetic approach and molecular markers, as performed in this study, is the best way to distinguish endophytic strains of Diaporthe isolated from common bean (Phaseolus vulgaris L.). en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Journal of Applied Microbiology pt-BR
dc.relation.ispartofseries v. 120, n. 2, p. 388-401, Fevereiro 2016 pt-BR
dc.rights The Society for Applied Microbiology pt-BR
dc.subject Diaporthe pt-BR
dc.subject DNA fingerprinting pt-BR
dc.subject Endophytic fungus pt-BR
dc.subject Genetic variability pt-BR
dc.subject IRAP pt-BR
dc.subject MLST pt-BR
dc.subject REMAP pt-BR
dc.title High genetic variability in endophytic fungi from the genus Diaporthe isolated from common bean (Phaseolus vulgaris L.) in Brazil en
dc.type Artigo pt-BR


Files in this item

This item appears in the following Collection(s)

  • Artigos [268]
    Artigos Técnico-científicos na área de Microbiologia

Show simple item record

Search DSpace


Browse

My Account