Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19685
Tipo: Artigo
Título: Investigation of genes involved in nisin production in Enterococcus spp. strains isolated from raw goat milk
Autor(es): Perin, Luana Martins
Todorov, Svetoslav Dimitrov
Nero, Luís Augusto
Abstract: Different strains of Lactococcus lactis are capable of producing the bacteriocin nisin. However, genetic transfer mechanisms allow the natural occurrence of genes involved in nisin production in members of other bacterial genera, such as Enterococcus spp. In a previous study, nisA was identified in eight enterococci capable of producing antimicrobial substances. The aim of this study was to verify the presence of genes involved in nisin production in Enterococcus spp. strains, as well as nisin expression. The nisA genes from eight Enterococcus spp. strains were sequenced and the translated amino acid sequences were compared to nisin amino-acid sequences previously described in databases. Although containing nisin structural and maturation related genes, the enterococci strains tested in the present study did not present the immunity related genes (nisFEG and nisI). The translated sequences of nisA showed some point mutations, identical to those presented by Lactococcus strains isolated from goat milk. All enterococci were inhibited by nisin, indicating the absence of immunity and thus that nisin cannot be expressed. This study demonstrated for the first time the natural occurrence of nisin structural genes in Enterococcus strains and highlights the importance of providing evidence of a link between the presence of bacteriocin genes and their expression.
Palavras-chave: Enterococcus
Nisin
Genetic mutation
Gene transfer
Editor: Antonie van Leeuwenhoek
Tipo de Acesso: Springer International Publishing Switzerland
URI: https://doi.org/10.1007/s10482-016-0721-6
http://www.locus.ufv.br/handle/123456789/19685
Data do documento: 2-Jun-2016
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