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|Título:||Follicular dynamics and gene expression in granulosa cells, corpora lutea and oocytes from gilts of breeds with low and high ovulation rates|
|Autor(es):||Silva, P. V.|
Guimarães, S. E. F.
Guimarães, J. D.
Nascimento, C. S.
Lopes, P. S.
Siqueira, J. B.
Amorim, L. S.
Silva, F. Fonseca e
Foxcroft, G. R.
|Abstract:||Follicular dynamics and the expression of candidate genes using real-time polymerase chain reaction (PCR) were compared during the oestrous cycle of pig breeds with high (commercial line; n = 24) and low (local Brazilian Piau; n = 21) ovulation rates and prolificacy. Gilts were killed on Days 0, 4, 10 and 18 of the oestrous cycle and visible ovarian follicles were classified by follicular diameter. Recovered cumulus–oocyte complexes were classified as normal or atretic and frozen in liquid nitrogen until RNA extraction. Low ovulation rates and/or prolificacy in Piau gilts was associated with a different pattern of follicle development, with lower numbers of small follicles on Day 18, fewer large follicles on Days 0 and 18 (P ≤ 0.05) and a higher proportion of atretic follicles on Days 0 and 18 (P ≤ 0.05). Compared with commercial line gilts, less-prolific Piau gilts exhibited higher expression of apoptotic genes during luteolysis (CASP3 and FASL; P ≤ 0.05), decreased expression of TGFBR2 and BAX mRNA in the corpus luteum (P ≤ 0.05), higher expression of apoptotic genes (FAS, BCL2 and CASP8; P ≤ 0.05) in granulosa cells and a greater abundance (P ≤ 0.05) of genes controlling oocyte-secreted factors (GDF9, BMP15 and BMP6), suggesting underlying mechanisms controlling differences in follicular development, ovulation rate and inherent prolificacy in this pig breed.|
|Editor:||Reproduction, Fertility and Development|
|Tipo de Acesso:||CSIRO|
|Data do documento:||7-Mar-2013|
|Aparece nas coleções:||Artigos|
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