Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/21406
Tipo: Artigo
Título: Comparative study of different molecular markers for classifying and establishing genetic relationships in Coffea canephora
Autor(es): Ferrão, Luís Felipe V.
Caixeta, Eveline T.
Souza, Flávio de F.
Zambolim, Eunize M.
Cruz, Cosme D.
Zambolim, Laércio
Sakiyama, Ney S.
Abstract: The genetic variability characterization of the accessions of the germplasm collection, using molecular markers, is being applied as a complementary strategy to the traditional approaches to redefine the plant genetic resources. In this study, we compared the informativeness and efficiency of the molecular markers RAPD, AFLP and SSR in the analysis of 94 accessions of Coffea canephora germplasm held by the breeding program of the Brazilian Agricultural Research Corporation (Embrapa), Rondônia State, Brazil. For this, we considered the marker’s discriminatory power and level of polymorphism detected and also the genetic relationships and clustering (dendrogram) analysis. The RAPD marker yielded low-quality data and problems in the discrimination of some accessions, being less recommended for genetic studies of C. canephora. The SSRs had a higher level of information content and yielded high-quality data, while AFLP was the most efficient marker system because of the simultaneous detection of abundant polymorphism markers per few reactions. Our results indicate that AFLP and SSR, allies to the intrinsic characteristics of each technique, are the most suitable molecular markers for genetic studies of C. canephora. However, the choice of AFLP or SSR in the species characterization should be made in agreement with some characteristics that are discussed in this work.
Palavras-chave: SSR
AFLP
RAPD
Genetic relationships
Robusta coffee
Editor: Plant Systematics and Evolution
Tipo de Acesso: Springer-Verlag Wien
URI: https://doi.org/10.1007/s00606-012-0717-2
http://www.locus.ufv.br/handle/123456789/21406
Data do documento: 3-Nov-2012
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
artigo.pdf
  Until 2100-12-31
Texto completo873,67 kBAdobe PDFVisualizar/Abrir    Solictar uma cópia


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.