Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/21527
Tipo: Artigo
Título: Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene
Autor(es): Brito, Giovani Greigh de
Caixeta, Eveline Teixeira
Gallina, Ana Paula
Zambolim, Eunize Maciel
Zambolim, Laércio
Diola, Valdir
Loureiro, Marcelo Elhers
Abstract: The most important disease of Coffea arabica is coffee leaf rust caused by the fungus Hemileia vastatrix. The purpose of this study was to characterize the inheritance of coffee resistance gene(s) to race II of this pathogen and to identify and map molecular markers linked to this trait. Different populations were used: F2 (160 plants), BCr (20), and BCs (135), derived from a cross between the resistant genotype Híbrido de Timor UFV 427-15 and the susceptible cultivar Catuaí Amarelo UFV 2143-236 (IAC 30). The segregation analysis showed that the resistance of Híbrido de Timor to race II of the H. vastatrix is conferred by a single dominant gene. The amplification of 176 AFLP (Amplified fragment length polymorphism) primer combinations using bulked segregant analysis (BSA) allowed the identification of three molecular markers linked to the resistance gene. Genetic mapping of these three markers in the F2 population indicated that they are distributed on both sides, flanking the resistance gene. The markers E.CTC/M.TTT405 and E.CGT/M.TGT300 were found linked to the resistance gene at 8.69 cM (LOD 18.91) and 25.10 cM (LOD 5.37), respectively, while E.CCT/M.TTC230 was localized on the other side of the gene, at 20.50 cM (LOD 6.15). These markers are the first rust resistance markers identified in Híbrido de Timor and can be useful for marker assisted selection in coffee breeding programs.
Palavras-chave: Coffee leaf rust
Resistance gene
AFLP marker
Bulked segregant analysis
Editor: Euphytica
Tipo de Acesso: Springer Science+Business Media B.V.
URI: https://doi.org/10.1007/s10681-010-0119-x
http://www.locus.ufv.br/handle/123456789/21527
Data do documento: Mar-2010
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