Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/22642
Tipo: Artigo
Título: Molecular mapping of the viral determinants of systemic wilting induced by a Lettuce mosaic virus (LMV) isolate in some lettuce cultivars
Autor(es): Zerbini, F. Murilo
Krause-Sakate, Renate
Redondo, Elise
Richard-Forget, Florence
Jadão, Adriana Salomão
German-Retana, Sylvie
Pavan, Marcelo Agenor
Candresse, Thierry
Gall, Olivier Le
Houvenaghel, Marie Christine
Abstract: The isolate AF199 of Lettuce mosaic virus (LMV, genus Potyvirus) causes local lesions followed by systemic wilting and plant death in the lettuce cultivars Ithaca and Vanguard 75. Analysis of the phenotype of virus chimeras revealed that a region within the P1 protein coding region (nucleotides 112–386 in the viral genome) and/or another one within the CI protein coding region (nucleotides 5496–5855) are sufficient together to cause the lethal wilting in Ithaca, but not in Vanguard 75. This indicates that the determinants of this particular symptom are different in these two lettuce cultivars. The wilting phenotype was not directly correlated with differences in the deduced amino acid sequence of these two regions. Furthermore, transient expression of the LMV-AF199 proteins, separately or in combination, did not induce local necrosis or any other visible reaction in the plants. Together, these results suggest that the systemic wilting reaction might be due to RNA rather than protein sequences.
Palavras-chave: Lactuca sativa
Agroinfiltration
Infectious cDNA
Necrosis
Potyvirus
Editor: Virus Research
Tipo de Acesso: 2004 Elsevier B.V. All rights reserved.
URI: https://doi.org/10.1016/j.virusres.2004.12.001
http://www.locus.ufv.br/handle/123456789/22642
Data do documento: Mai-2005
Aparece nas coleções:Fitopatologia - Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
artigo.pdf
  Until 2100-12-31
texto completo237,48 kBAdobe PDFVisualizar/Abrir ACESSO RESTRITO


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.