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https://locus.ufv.br//handle/123456789/22642
Tipo: | Artigo |
Título: | Molecular mapping of the viral determinants of systemic wilting induced by a Lettuce mosaic virus (LMV) isolate in some lettuce cultivars |
Autor(es): | Zerbini, F. Murilo Krause-Sakate, Renate Redondo, Elise Richard-Forget, Florence Jadão, Adriana Salomão German-Retana, Sylvie Pavan, Marcelo Agenor Candresse, Thierry Gall, Olivier Le Houvenaghel, Marie Christine |
Abstract: | The isolate AF199 of Lettuce mosaic virus (LMV, genus Potyvirus) causes local lesions followed by systemic wilting and plant death in the lettuce cultivars Ithaca and Vanguard 75. Analysis of the phenotype of virus chimeras revealed that a region within the P1 protein coding region (nucleotides 112–386 in the viral genome) and/or another one within the CI protein coding region (nucleotides 5496–5855) are sufficient together to cause the lethal wilting in Ithaca, but not in Vanguard 75. This indicates that the determinants of this particular symptom are different in these two lettuce cultivars. The wilting phenotype was not directly correlated with differences in the deduced amino acid sequence of these two regions. Furthermore, transient expression of the LMV-AF199 proteins, separately or in combination, did not induce local necrosis or any other visible reaction in the plants. Together, these results suggest that the systemic wilting reaction might be due to RNA rather than protein sequences. |
Palavras-chave: | Lactuca sativa Agroinfiltration Infectious cDNA Necrosis Potyvirus |
Editor: | Virus Research |
Tipo de Acesso: | 2004 Elsevier B.V. All rights reserved. |
URI: | https://doi.org/10.1016/j.virusres.2004.12.001 http://www.locus.ufv.br/handle/123456789/22642 |
Data do documento: | Mai-2005 |
Aparece nas coleções: | Fitopatologia - Artigos |
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artigo.pdf Until 2100-12-31 | texto completo | 237,48 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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