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https://locus.ufv.br//handle/123456789/22643
Tipo: | Artigo |
Título: | Revisiting the classification of curtoviruses based on genome-wide pairwise identity |
Autor(es): | Zerbini, F. Murilo Varsani, Arvind Martin, Darren P. Navas-Castillo, Jesús Moriones, Enrique Hernández-Zepeda, Cecilia Idris, Ali Brown, Judith K. |
Abstract: | Members of the genus Curtovirus (family Geminiviridae) are important pathogens of many wild and cultivated plant species. Until recently, relatively few full curtovirus genomes have been characterised. However, with the 19 full genome sequences now available in public databases, we revisit the proposed curtovirus species and strain classification criteria. Using pairwise identities coupled with phylogenetic evidence, revised species and strain demarcation guidelines have been instituted. Specifically, we have established 77 % genome-wide pairwise identity as a species demarcation threshold and 94 % genome-wide pairwise identity as a strain demarcation threshold. Hence, whereas curtovirus sequences with >77 % genome-wide pairwise identity would be classified as belonging to the same species, those sharing >94 % identity would be classified as belonging to the same strain. We provide step-by-step guidelines to facilitate the classification of newly discovered curtovirus full genome sequences and a set of defined criteria for naming new species and strains. The revision yields three curtovirus species: Beet curly top virus (BCTV), Spinach severe surly top virus (SpSCTV) and Horseradish curly top virus (HrCTV). |
Palavras-chave: | Full genome sequence Pairwise identity Demarcation criterion Pairwise sequence alignment Species demarcation |
Editor: | Archives of Virology |
Tipo de Acesso: | Springer-Verlag Wien 2014 |
URI: | http://dx.doi.org/10.1007/s00705-014-1982-x http://www.locus.ufv.br/handle/123456789/22643 |
Data do documento: | 25-Jan-2014 |
Aparece nas coleções: | Fitopatologia - Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | texto completo | 460,23 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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