Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/22719
Tipo: Artigo
Título: Pattern of protein expression in the epididymis of Oligoryzomys nigripes (Cricetidae, Sigmodontinae)
Autor(es): Menezes, Tatiana Prata
Hill, Eric
Moura, Arlindo de Alencar
Lobo, Marina D. P.
Breton, Sylvie
Machado-Neves, Mariana
Monteiro-Moreira, Ana Cristina O.
Abstract: In the epididymis, epithelial cells work in a concerted manner to create a luminal environment for sperm maturation, transport, and storage. However, the cell functions may be affected by anthropogenic factors, causing negative impacts on male fertility. In our study, we describe the pattern of protein expression in the epithelium and luminal fluid from epididymis of Oligoryzomys nigripes, a South American sigmodontine rodent whose reproductive biology has been little studied. Nine animals were captured from a preserved area of Atlantic Forest, where the exposure to anthropogenic influences is minimal. Epididymides were processed for histological analysis under light and epifluorescence microscopy, in which we used cell-specific markers aquaporin 9 (AQP9), vacuolar H+-ATPase (V-ATPase), and cytokeratin 5 (KRT5). Other samples were assessed for protein expression using shotgun proteomics. Similar to laboratory rodents, principal cells expressed AQP9 in their stereocilia. Basal cells, identified by KRT5 labeling, presented lateral body projections and a few axiopodia going toward the lumen. Clear cells expressed V-ATPase in their sub-apical vesicles and microplicae, and showed different shapes along the duct. Shotgun proteomics detected 51 proteins from epididymal supernatant. Most of them have been previously described in other species, indicating that they are well conserved. Twenty-three proteins detected in O. nigripes have not been described in epididymis from other South American sigmodontine rodents, confirming that the secretion pattern is species-specific. Our findings in O. nigripes from a protected area may help to create a baseline for studies investigating the effects of anthropogenic factors on functionality of the epididymal epithelium
Palavras-chave: Epithelial cells
Epididymal fluid
Proteome
Morphometry
Reproductive biology
Editor: Cell and Tissue Research
Tipo de Acesso: Springer-Verlag GmbH Germany 2017
URI: https://doi.org/10.1007/s00441-017-2714-9
http://www.locus.ufv.br/handle/123456789/22719
Data do documento: Abr-2018
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