Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/22927
Tipo: Artigo
Título: Differentially transcribed genes in skeletal muscle of lambs
Autor(es): Lôbo, Ana Maria Bezerra Oliveira
Guimarães, Simone Eliza Faccioni
Paiva, Samuel Rezende
Cardoso, Fernando Flores
Silva, Fabyano Fonseca
Fernandes Júnior, Gerardo Alves
Lôbo, Raimundo Nonato Braga
Abstract: The objective of this study was to compare gene transcription profiles in Longissimus dorsi muscle of the following four hair sheep genetic groups, Morada Nova (MO), Brazilian Somali (SO), Santa Inˆes (SI) and 1⁄2Dorper  1⁄2Morada Nova (F1). These groups all display different postnatal muscle growth. The transcriptomes of the skeletal muscle of the lambs (at 200 days of age) were profiled by using oligonucleotide microarrays and reverse transcription- quantitative real-time PCR (RT-qPCR). The microarray experiment identified 262 transcripts that were differentially expressed when transcription levels were compared between the different breeds. A total of 23 transcripts among which those involved in skeletal muscle development (MyoD1 and IGFBP4), lipogenesis and adipogenesis (C/EBP d , PPAR g and PGDS) were differentially expressed in at least in one comparison. Clustering analysis showed that there is greater similarity in gene expression between the MO and SI breeds and between F1 and SO genetic groups. The SO breed has the most distinct expression pattern. The RT-qPCR results confirmed the findings from the microarray study. A positive correlation was observed between the expression of MyoD1 and the cold carcass yield. The negative correlations between the weight and yield of cold carcass with the expression of C/EBP d mean that the selection for adipogenesis could lead to a lower carcass weight. The GLUT3 and PYGL gene transcripts were negatively correlated with fat thickness, but ATP5G1 was positively correlated with this trait. Interestingly, many genes negatively correlated with PUFA were positively correlated with cold carcass yield. In conclusion, the present work demonstrated that there are breed-specific expression patterns in Brazilian hair sheep genetic groups. The differences in gene expression among genetic groups were consistent with their phenotypic differences. The positive correlation of the MyoD1 expression with the cold carcass yield suggests that this gene is important for tissue growth in sheep. Thepositive correlation of the C/EBP d expression with PUFA provides an opportunity to select for lipid deposition in meat animals.
Palavras-chave: Cluster
Differential expression
IGFBP4
MyoD1
Ovis aries
Editor: Livestock Science
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1016/j.livsci.2012.07.027
http://www.locus.ufv.br/handle/123456789/22927
Data do documento: Dez-2012
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