Flow cytometry and cytogenetic tools in eucalypts: genome size variation × karyotype stability

Show simple item record Carvalho, Guilherme Mendes Almeida Carvalho, Carlos Roberto Soares, Fernanda Aparecida Ferrari 2019-01-25T12:37:34Z 2019-01-25T12:37:34Z 2017-10
dc.identifier.issn 1614-2950
dc.description.abstract The eucalypts comprise a group of woody plants used in commercial forest plantations owing to their high growth rates, adaptability to various ecological conditions and multiple applications. Despite the enormous amount of molecular data available for eucalypts, a basic understanding of the nature of its genome still requires information regarding the DNA amount in the genus. In this work, we estimated the genome size and base composition of 25 eucalypt species. With a comparative karyotype approach, we aimed to identify possible chromosomal alterations correlated with the genome size variation. Classical cytogenetic and genomic in situ hybridization experiments were conducted for this purpose. The studied species showed genome size ranging from 2C = 0.91 (Corymbia intermedia) to 2C = 1.37 pg (Eucalyptus paniculata) and AT/CG ratios varying from AT = 61.3 (Eucalyptus urophylla) to AT = 62.85% (C. intermedia). Comparative karyotype analysis revealed no remarkable differences in chromosome number (2n = 22) or morphology among eucalypt species despite considerable differences in nuclear DNA content. The genome in situ hybridization method did not distinguish non-homologous chromosomal regions of Eucalyptus baileyana and Corymbia citriodora, despite the difference of 0.45 pg between their genome sizes. The results found in the present work corroborate the consideration of small and dispersed DNA changes as the main cause of genome size variation in eucalypts. en
dc.format pdf pt-BR
dc.language.iso eng pt-BR
dc.publisher Tree Genetics & Genomes pt-BR
dc.relation.ispartofseries Volume 13, Issue 5, October 2017 pt-BR
dc.rights Springer-Verlag GmbH Germany 2017 pt-BR
dc.subject Eucalyptus pt-BR
dc.subject Corymbia pt-BR
dc.subject 2C-value pt-BR
dc.subject Base composition pt-BR
dc.subject Chromosome pt-BR
dc.subject Karyotype pt-BR
dc.title Flow cytometry and cytogenetic tools in eucalypts: genome size variation × karyotype stability en
dc.type Artigo pt-BR

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    Artigos Técnico-científicos na área de Biologia Geral

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