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https://locus.ufv.br//handle/123456789/23272
Tipo: | Artigo |
Título: | De novo domestication of wild tomato using genome editing |
Autor(es): | Zsögön, Agustin Čermák, Tomáš Naves, Emmanuel Rezende Notini, Marcela Morato Edel, Kai H. Weinl, Stefan Freschi, Luciano Voytas, Daniel F. Kudla, Jörg Peres, Lázaro Eustáquio Pereira |
Abstract: | Breeding of crops over millennia for yield and productivity1 has led to reduced genetic diversity. As a result, beneficial traits of wild species, such as disease resistance and stress tolerance, have been lost2. We devised a CRISPR–Cas9 genome engineering strategy to combine agronomically desirable traits with useful traits present in wild lines. We report that editing of six loci that are important for yield and productivity in present-day tomato crop lines enabled de novo domestication of wild Solanum pimpinellifolium. Engineered S. pimpinellifolium morphology was altered, together with the size, number and nutritional value of the fruits. Compared with the wild parent, our engineered lines have a threefold increase in fruit size and a tenfold increase in fruit number. Notably, fruit lycopene accumulation is improved by 500% compared with the widely cultivated S. lycopersicum. Our results pave the way for molecular breeding programs to exploit the genetic diversity present in wild plants. |
Palavras-chave: | Wild tomato Genome editing Domestication |
Editor: | Nature Biotechnology |
Tipo de Acesso: | Open Access |
URI: | http://dx.doi.org/10.1038/nbt.4272 http://www.locus.ufv.br/handle/123456789/23272 |
Data do documento: | Dez-2018 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf | texto completo | 5,29 MB | Adobe PDF | Visualizar/Abrir |
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