Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/23272
Tipo: Artigo
Título: De novo domestication of wild tomato using genome editing
Autor(es): Zsögön, Agustin
Čermák, Tomáš
Naves, Emmanuel Rezende
Notini, Marcela Morato
Edel, Kai H.
Weinl, Stefan
Freschi, Luciano
Voytas, Daniel F.
Kudla, Jörg
Peres, Lázaro Eustáquio Pereira
Abstract: Breeding of crops over millennia for yield and productivity1 has led to reduced genetic diversity. As a result, beneficial traits of wild species, such as disease resistance and stress tolerance, have been lost2. We devised a CRISPR–Cas9 genome engineering strategy to combine agronomically desirable traits with useful traits present in wild lines. We report that editing of six loci that are important for yield and productivity in present-day tomato crop lines enabled de novo domestication of wild Solanum pimpinellifolium. Engineered S. pimpinellifolium morphology was altered, together with the size, number and nutritional value of the fruits. Compared with the wild parent, our engineered lines have a threefold increase in fruit size and a tenfold increase in fruit number. Notably, fruit lycopene accumulation is improved by 500% compared with the widely cultivated S. lycopersicum. Our results pave the way for molecular breeding programs to exploit the genetic diversity present in wild plants.
Palavras-chave: Wild tomato
Genome editing
Domestication
Editor: Nature Biotechnology
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1038/nbt.4272
http://www.locus.ufv.br/handle/123456789/23272
Data do documento: Dez-2018
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